Jahnsen H, Kristensen B W, Thiébaud P, Noraberg J, Jakobsen B, Bove M, Martinoia S, Koudelka-Hep M, Grattarola M, Zimmer J
Division of Neurophysiology, Department of Medical Physiology, University of Copenhagen, Blegdamsvej 3, Copenhagen N, DK-2200, Denmark.
Methods. 1999 Jun;18(2):160-72. doi: 10.1006/meth.1999.0769.
Fetal or early postnatal brain tissue can be cultured in viable and healthy condition for several weeks with development and preservation of the basic cellular and connective organization as so-called organotypic brain slice cultures. Here we demonstrate and describe how it is possible to establish such hippocampal rat brain slice cultures on biocompatible silicon-based chips with arrays of electrodes with a histological organization comparable to that of conventional brain slice cultures grown by the roller drum technique and on semiporous membranes. Intracellular and extracellular recordings from neurons in the slice cultures show that the electroresponsive properties of the neurons and synaptic circuitry are in accordance with those described for cells in acutely prepared slices of the adult rat hippocampus. Based on the recordings and the possibilities of stimulating the cultured cells through the electrode arrays it is anticipated that the setup eventually will allow long-term studies of defined neuronal networks and provide valuable information on both normal and neurotoxicological and neuropathological conditions.
胎儿或出生后早期的脑组织可以在存活且健康的状态下培养数周，其基本细胞和结缔组织得以发育和保存，即所谓的器官型脑片培养。在此，我们展示并描述了如何在具有电极阵列的生物相容性硅基芯片上建立这种大鼠海马脑片培养物，其组织学结构与通过滚筒技术在半透膜上培养的传统脑片培养物相当。从脑片培养物中的神经元进行的细胞内和细胞外记录表明，神经元的电反应特性和突触回路与成年大鼠海马急性制备切片中的细胞所描述的一致。基于这些记录以及通过电极阵列刺激培养细胞的可能性，预计该装置最终将允许对特定神经元网络进行长期研究，并提供有关正常、神经毒理学和神经病理学状况的有价值信息。
