Iwasaki Y, Nakabayashi N, Nakatani M, Mihara T, Kurita K, Ishihara K
Institute for Medical and Dental Engineering, Tokyo Medical and Dental University, Japan.
J Biomater Sci Polym Ed. 1999;10(5):513-29. doi: 10.1163/156856299x00450.
The competitive adsorption of proteins and phospholipids on omega-methacryloyloxyalkyl phosphorylcholine (MAPC) polymer was evaluated in this study. Albumin, fibrinogen, and dimyrstoyl phosphatidylcholine (DMPC) were used as model components. The amount of DMPC adsorbed on the MAPC polymers increased with an increase in the MAPC unit composition of the polymer. The methylene chain length of the MAPC unit was another factor influencing the DMPC adsorption when the MAPC unit composition of the MAPC polymer was low. The state of albumin and DMPC liposome adsorbed on the 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer was determined by dynamic contact angle (DCA) measurement. The adsorption strength of albumin on the MPC polymer was weaker than that on the poly[n-butyl methacrylate (BMA)], that is, the albumin was detached from the MPC polymer during the rinsing process. On the poly(BMA) surface, no difference in the shape of the DCA loops before and after contact with the DMPC liposomal suspension was observed. Fibrinogen adsorption on the MAPC polymer was detected by gold-colloid labeled immunoassay. The amount of fibrinogen adsorbed on every MAPC polymer surface was reduced by addition of the DMPC liposome in the fibrinogen solution. The number of platelets adhered on the MAPC polymer was also decreased when the DMPC liposome was present in the fibrinogen solution during pretreatment. We concluded that phospholipids were preferentially adsorbed on the MAPC polymer surface compared with plasma protein and that the adsorbed phospholipids played an important role in showing an excellent blood compatibility on the MAPC polymer.
本研究评估了蛋白质和磷脂在ω-甲基丙烯酰氧基烷基磷酰胆碱（MAPC）聚合物上的竞争性吸附。使用白蛋白、纤维蛋白原和二肉豆蔻酰磷脂酰胆碱（DMPC）作为模型成分。吸附在MAPC聚合物上的DMPC量随聚合物中MAPC单元组成的增加而增加。当MAPC聚合物的MAPC单元组成较低时，MAPC单元的亚甲基链长度是影响DMPC吸附的另一个因素。通过动态接触角（DCA）测量确定吸附在2-甲基丙烯酰氧基乙基磷酰胆碱（MPC）聚合物上的白蛋白和DMPC脂质体的状态。白蛋白在MPC聚合物上的吸附强度比在聚甲基丙烯酸正丁酯（BMA）上弱，也就是说，在冲洗过程中白蛋白从MPC聚合物上脱离。在聚（BMA）表面，与DMPC脂质体悬浮液接触前后DCA环的形状没有差异。通过金胶体标记免疫测定法检测纤维蛋白原在MAPC聚合物上的吸附。在纤维蛋白原溶液中加入DMPC脂质体后，吸附在每个MAPC聚合物表面的纤维蛋白原量减少。当在预处理过程中纤维蛋白原溶液中存在DMPC脂质体时，粘附在MAPC聚合物上的血小板数量也会减少。我们得出结论，与血浆蛋白相比，磷脂优先吸附在MAPC聚合物表面，并且吸附的磷脂在MAPC聚合物表现出优异的血液相容性方面起着重要作用。
