Castellón E A, Huidobro C C
Physiology and Biophysics Program, Institute of Biomedical Sciences, Faculty of Medicine, University of Chile, PO Box 70005, Postal Code 6530499, Santiago, Chile.
Hum Reprod. 1999 Jun;14(6):1522-7. doi: 10.1093/humrep/14.6.1522.
The human epididymis and its secretions actively promote sperm fertilizing capacity and provide protection for spermatozoa against harmful influences. Among epididymal secretions, glycosidases have been recently studied and associated with molecular changes on the sperm surface. In the present work, we studied the influence of different concentrations of testosterone, dihydrotestosterone and cyproterone acetate on the secretion of alpha-glucosidase, N-acetyl-glucosaminidase, beta-glucuronidase and alpha-mannosidase by isolated and cultured epithelial cells from human caput, corpus and cauda epididymides. Cell cultures were obtained from aggregates of isolated tubule fragments plated on extracellular matrix-covered multi-well plates. Activities of the glycosidases were measured in conditioned culture media and were higher in the distal regions of the epididymis. Testosterone and dihydrotestosterone significantly increase the enzyme secretion in a concentration-dependent manner. This increase was higher in corpus and/or cauda than in caput epididymis. Cyproterone acetate caused a dose-dependent decrease in glycosidase secretion in cultures from all epididymal regions. It is concluded that the secretion of epididymal glycosidases is regulated by androgen, being stimulated by dihydrotestosterone and testosterone and inhibited by the androgen antagonist cyproterone acetate.
人类附睾及其分泌物能积极促进精子的受精能力，并为精子提供保护，使其免受有害影响。在附睾分泌物中，糖苷酶最近已被研究，且与精子表面的分子变化有关。在本研究中，我们研究了不同浓度的睾酮、双氢睾酮和醋酸环丙孕酮对从人附睾头、体和尾分离培养的上皮细胞分泌α-葡萄糖苷酶、N-乙酰葡糖胺酶、β-葡萄糖醛酸酶和α-甘露糖苷酶的影响。细胞培养物是从接种在覆盖有细胞外基质的多孔板上的分离小管片段聚集体中获得的。在条件培养基中测量糖苷酶的活性，其在附睾的远端区域较高。睾酮和双氢睾酮以浓度依赖的方式显著增加酶的分泌。这种增加在附睾体和/或附睾尾比在附睾头更高。醋酸环丙孕酮导致所有附睾区域培养物中糖苷酶分泌呈剂量依赖性降低。得出的结论是，附睾糖苷酶的分泌受雄激素调节，被双氢睾酮和睾酮刺激，被雄激素拮抗剂醋酸环丙孕酮抑制。
