Vailhé B, Dietl J, Kapp M, Toth B, Arck P
Université Joseph Fourier, Grenoble, France.
Hum Reprod. 1999 Jun;14(6):1628-34. doi: 10.1093/humrep/14.6.1628.
Spontaneous pregnancy loss affects 15-18% of couples, and a number of potential causes are being discussed. The purpose of the present study was to assess if angiogenic disorders in the decidua of early human pregnancy could be related to spontaneous abortions. First trimester human decidua from elective terminations of normally progressing pregnancies and from missed abortions were investigated immunohistochemically. We quantified vessel density in decidua from normal pregnancies and from abortions by von Willebrand factor (vWF), platelet endothelial cell adhesion molecule (PECAM-1) and CD34 staining. Decidual blood vessel expression of alphavbeta3 integrin was also investigated. Significant increase (P < 0.02) in vessel density was observed in decidua parietalis of abortions, compared to decidua basalis. This increase was detected on slides stained for vWF and CD34, but not for PECAM-1. We observed a 15% increase analysing with vWF and a 77% increase with CD34 staining. alphavbeta3 integrin expression was not significantly different, neither in decidua parietalis from abortion, nor parietalis from normal pregnancies. Our data suggest that the increased vascularization in decidua parietalis from abortions could reflect complex disorders, such as specific cytokine expressions and hypoxia phenomena during the development of the decidua.
自然流产影响15%至18%的夫妇，目前正在探讨一些潜在原因。本研究的目的是评估人类早期妊娠蜕膜中的血管生成紊乱是否与自然流产有关。对正常妊娠选择性终止妊娠和稽留流产的孕早期人类蜕膜进行免疫组织化学研究。我们通过血管性血友病因子（vWF）、血小板内皮细胞黏附分子（PECAM-1）和CD34染色对正常妊娠和流产蜕膜中的血管密度进行定量。还研究了蜕膜血管中αvβ3整合素的表达。与基蜕膜相比，流产者壁蜕膜中的血管密度显著增加（P<0.02）。这种增加在vWF和CD34染色的切片上检测到，但在PECAM-1染色的切片上未检测到。用vWF分析时我们观察到增加了15%，用CD34染色时增加了77%。αvβ3整合素表达在流产者的壁蜕膜和正常妊娠的壁蜕膜中均无显著差异。我们的数据表明，流产者壁蜕膜中血管化增加可能反映了复杂的紊乱，如蜕膜发育过程中的特定细胞因子表达和缺氧现象。
