Damle R G, Yeolekar L R, Rao B L
National Institute of Virology, Maharashtra, India.
Acta Virol. 1998 Dec;42(6):389-95.
Monoclonal antibodies (MAbs) against an Indian strain (804994) and an Egyptian strain (E 101) of West Nile virus (WNV) were prepared in mice. Nine MAbs against the 804994 strain and 5 MAbs against E 101 strain were obtained. All 14 MAbs reacted with the envelope (E) protein of WNV in an immunoblot assay. They were tested by an enzyme-linked immunosorbent assay (ELISA) for their cross-reactivity with WNV, Japanese encephalitis virus (JEV) and Dengue-2 virus (DEN-2), and for their reactivity in haemagglutination-inhibition (HAI) test. Based on these results MAbs were broadly grouped into three groups, namely WNV-specific HAI-positive, WNV-JEV cross-reactive HAI-positive, and WNV-JEV cross-reactive HAI-negative MAbs. The antigenic cross-reactivity between twelve WNV strains isolated from different geographical regions and their respective hosts was assessed using these MAbs in HAI and complement fixation (CF) tests. The strain analysis by CF distinguished Indian from South African strains. However, a similarity between some Indian and South African strains in HAI was observed. E 101 strain appeared to have antigenic similarity with Indian as well as South African strains. Overall it appears that antigenically similar strains of WNV are prevalent in India. A single heterogenous domain was apparent on the epitope map of WNV deduced by ELISA additivity test.
在小鼠体内制备了针对西尼罗河病毒（WNV）印度株（804994）和埃及株（E 101）的单克隆抗体（MAb）。获得了9种针对804994株的单克隆抗体和5种针对E 101株的单克隆抗体。在免疫印迹试验中，所有14种单克隆抗体均与WNV的包膜（E）蛋白发生反应。通过酶联免疫吸附测定（ELISA）检测它们与WNV、日本脑炎病毒（JEV）和登革热2型病毒（DEN-2）的交叉反应性，以及它们在血凝抑制（HAI）试验中的反应性。基于这些结果，单克隆抗体大致分为三组，即WNV特异性HAI阳性、WNV-JEV交叉反应性HAI阳性和WNV-JEV交叉反应性HAI阴性单克隆抗体。使用这些单克隆抗体在HAI和补体结合（CF）试验中评估了从不同地理区域及其各自宿主分离的12株WNV菌株之间的抗原交叉反应性。通过CF进行的菌株分析区分了印度菌株和南非菌株。然而，观察到一些印度菌株和南非菌株在HAI方面存在相似性。E 101株似乎与印度菌株以及南非菌株具有抗原相似性。总体而言，WNV抗原相似的菌株在印度很普遍。通过ELISA加性试验推导的WNV表位图谱上出现了一个单一的异质结构域。
