Triki H, Bahri O, Guillot S, VAN DER Avoort H G A M, Ben Sassi J, Arrouji A, Arrouji Z, Slim A, Crainic R, Dellagi K
WHO Regional Reference Laboratory on Poliomyelitis, Institut Pasteur, Tunis, Tunisia.
Unite de Virologie Medicale, Institut Pasteur, Paris, France.
J Med Microbiol. 1999 Jun;48(6):569-576. doi: 10.1099/00222615-48-6-569.
This report is an overview of poliomyelitis surveillance in Tunisia from 1991 to 1996. In all, 2088 stool specimens, collected from 152 acute flaccid paralysis (AFP) cases and from 1747 of their healthy contacts were investigated. Virus isolation was done systematically in RD and HEp-2C cell lines and isolated viruses were typed by sero-neutralisation as polioviruses or non-polio enteroviruses. Poliovirus isolates were analysed systematically for their wild or vaccine-related origin by two methods--one based on antigenic differences and one on genetic differences between strains. All type 2 polioviruses were vaccine-related and most wild viruses belonged to polio serotype 3. Wild polio type 3 viruses were detected in 1991 and 1992 in six cases of paralytic polio. A silent circulation of wild polio 1 and wild polio 3 was detected in 1994. No wild virus was detected in Tunisia from 1995 onwards. Wild polioviruses were sequenced and compared with Tunisian wild strains isolated during the 1980s, as well as other genotypes from the international database. These investigations revealed a single Tunisian polio 3 genotype that has been circulating from 1985 to 1994 and two different polio 1 genotypes. These results reflect effective control strategies within the country and contribute to the improvement of the polio eradication programme effectiveness at national and global levels.
本报告概述了1991年至1996年突尼斯的脊髓灰质炎监测情况。总共对从152例急性弛缓性麻痹（AFP）病例及其1747名健康接触者采集的2088份粪便标本进行了调查。在RD和HEp - 2C细胞系中系统地进行病毒分离，分离出的病毒通过血清中和法鉴定为脊髓灰质炎病毒或非脊髓灰质炎肠道病毒。通过两种方法系统分析脊髓灰质炎病毒分离株的野生型或疫苗相关型来源——一种基于抗原差异，另一种基于毒株间的基因差异。所有2型脊髓灰质炎病毒均与疫苗相关，大多数野生病毒属于脊髓灰质炎3型血清型。1991年和1992年在6例麻痹性脊髓灰质炎病例中检测到野生3型脊髓灰质炎病毒。1994年检测到野生1型和野生3型脊髓灰质炎病毒的隐性传播。1995年起在突尼斯未检测到野生病毒。对野生脊髓灰质炎病毒进行测序，并与20世纪80年代分离的突尼斯野生毒株以及国际数据库中的其他基因型进行比较。这些调查揭示了一种自1985年至1994年一直在传播的突尼斯脊髓灰质炎3型单一基因型和两种不同的脊髓灰质炎1型基因型。这些结果反映了该国有效的控制策略，并有助于提高国家和全球层面脊髓灰质炎根除计划的成效。
