Kalsow C M, Dubielzig R R, Dwyer A E
Department of Ophthalmology, University of Rochester School of Medicine and Dentistry, New York 14642, USA.
Invest Ophthalmol Vis Sci. 1999 Jun;40(7):1611-5.
Pinealitis accompanying uveitis is well established in laboratory models of experimental autoimmune uveoretinitis. In naturally occurring uveitis, pinealitis has been demonstrated in the pineal gland from a mare with active uveitis and is suspected in some human uveitides. We have evaluated pineal glands from horses with various stages of uveitis for signs of immunopathology accompanying spontaneous uveitis.
Pineal glands from 10 horses with uveitis and from 13 horses without uveitis were evaluated for histochemical (H&E, collagen) and immunohistochemical (MHC class II antigen expression, infiltration of T and B lymphocytes, and glial fibrillary acidic protein (GFAP) and vimentin upregulation) evidence of inflammation.
Septal areas of pineal glands from horses with uveitis had clusters of MHC class II antigen-expressing cells, T lymphocytes, and enhanced collagen deposition. These changes were not as readily observed in pineal glands from horses without uveitis. B lymphocytes were detected only in the pineal gland from the one mare with active uveitis in which T and B lymphocytes were organized into follicles. No differences in GFAP or vimentin immunoreactivity were noted in pineal glands from horses with or without uveitis.
These pineal gland changes suggest that the pinealitis associated with equine uveitis is transient just as the uveitis of these horses is recurrent. Study of pineal glands from horses with clinically documented uveitis allows demonstration of subtle pineal changes associated with natural uveitis. Similar changes would be difficult to document in human patient populations.
在实验性自身免疫性葡萄膜视网膜炎的实验室模型中，葡萄膜炎伴发松果体炎已得到充分证实。在自然发生的葡萄膜炎中，已在一匹患有活动性葡萄膜炎的母马的松果体中证实存在松果体炎，并且在一些人类葡萄膜炎病例中也怀疑有松果体炎。我们评估了患有不同阶段葡萄膜炎的马的松果体，以寻找伴随自发性葡萄膜炎的免疫病理学迹象。
对10匹患有葡萄膜炎的马和13匹未患葡萄膜炎的马的松果体进行组织化学（苏木精-伊红染色、胶原染色）和免疫组织化学（MHC II类抗原表达、T和B淋巴细胞浸润以及胶质纤维酸性蛋白（GFAP）和波形蛋白上调）炎症证据评估。
患有葡萄膜炎的马的松果体间隔区域有表达MHC II类抗原的细胞簇、T淋巴细胞，且胶原沉积增加。这些变化在未患葡萄膜炎的马的松果体中不太容易观察到。仅在一匹患有活动性葡萄膜炎的母马的松果体中检测到B淋巴细胞，其中T和B淋巴细胞组织成滤泡。在患有或未患葡萄膜炎的马的松果体中，未观察到GFAP或波形蛋白免疫反应性的差异。
这些松果体变化表明，与马葡萄膜炎相关的松果体炎是短暂的，就像这些马的葡萄膜炎会复发一样。对临床记录有葡萄膜炎的马的松果体进行研究，可以证明与自然葡萄膜炎相关的细微松果体变化。在人类患者群体中很难记录到类似的变化。
