Bobanovic L K, Laine M, Petersen C C, Bennett D L, Berridge M J, Lipp P, Ripley S J, Bootman M D
Laboratory of Molecular Signalling, The Babraham Institute, Babraham, Cambridge CB2 4AT, UK.
Biochem J. 1999 Jun 15;340 ( Pt 3)(Pt 3):593-9.
We report the sequence, structure and distribution of a novel transient receptor potential (trp) homologue from Xenopus, Xtrp, determined by screening an oocyte cDNA library. On the basis of sequence similarity and predicted structure, Xtrp appears to be a homologue of mammalian trp1 proteins. Two polyclonal antibodies raised against distinct regions of the Xtrp sequence revealed Xtrp expression in various Xenopus tissues, and the localization of Xtrp at the plasma membrane of Xenopus oocytes and HeLa cells. Since capacitative calcium entry into Xenopus oocytes has been shown previously to be substantially inhibited by trp1 antisense oligonucleotides [Tomita, Kaneko, Funayama, Kondo, Satoh and Akaike (1998) Neurosci. Lett. 248, 195-198] we suggest that Xtrp may underlie capacitative calcium entry in Xenopus tissues.
我们报告了通过筛选爪蟾卵母细胞cDNA文库确定的一种来自爪蟾的新型瞬时受体电位（trp）同源物Xtrp的序列、结构和分布。基于序列相似性和预测结构，Xtrp似乎是哺乳动物trp1蛋白的同源物。针对Xtrp序列不同区域产生的两种多克隆抗体揭示了Xtrp在爪蟾各种组织中的表达，以及Xtrp在爪蟾卵母细胞和HeLa细胞质膜上的定位。由于先前已表明trp1反义寡核苷酸可显著抑制钙池调控性钙内流进入爪蟾卵母细胞[富田、金子、船山、近藤、佐藤和赤池（1998年）《神经科学快报》248，195 - 198]，我们认为Xtrp可能是爪蟾组织中钙池调控性钙内流的基础。
