Petersen C C, Berridge M J, Borgese M F, Bennett D L
Babraham Institute Laboratory of Molecular Signalling, Department of Zoology, University of Cambridge, U.K.
Biochem J. 1995 Oct 1;311 ( Pt 1)(Pt 1):41-4. doi: 10.1042/bj3110041.
Capacitative calcium entry is a major pathway through which intracellular calcium stores are refilled after stimulation. It has been suggested that the protein encoded by the transient receptor potential (trp) gene expressed in Drosophila photoreceptors may be homologous with capacitative calcium entry channels. Expression of the trp gene product in Xenopus oocytes led to significant increases in calcium entry only when the intracellular calcium stores were depleted. Previous investigations have found trp to be uniquely expressed in Drosophila photoreceptors, but PCR cloning shows that homologous proteins exist in Calliphora, mouse brain and Xenopus oocytes. It is thus possible that capacitative calcium entry in Xenopus oocytes is mediated by a homologue of trp.
容量性钙内流是刺激后细胞内钙库重新充盈的主要途径。有人提出，果蝇光感受器中表达的瞬时受体电位（trp）基因编码的蛋白质可能与容量性钙内流通道同源。仅当细胞内钙库耗尽时，trp基因产物在非洲爪蟾卵母细胞中的表达才会导致钙内流显著增加。先前的研究发现trp仅在果蝇光感受器中特异性表达，但聚合酶链反应（PCR）克隆表明，丽蝇、小鼠脑和非洲爪蟾卵母细胞中存在同源蛋白。因此，非洲爪蟾卵母细胞中的容量性钙内流可能由trp的同源物介导。
