[Genetic polymorphism at FES locus in Chinese and German populations].

OBJECTIVE

The aim of this study on the genetic polymorphism at FES locus was to know whether there is genetic relationship between Chinese and German populations.

METHODS

EDTA-blood specimens were collected from 311 healthy unrelated Han individuals in Jilin, Chengdu and Guangzhou in China and from 123 healthy unrelated individuals in Germany. The DNA samples were extracted using Chelex method and amplified by PCR technique. The PAGE horizontal electrophoresis was used for typing the PCR product.DNA sequences were analyzed by ALF.

RESULTS

There were nine alleles at FES locus in the Chinese population. The allele frequencies were FES6,0-0. 0051; FES 8,0-0.0042; FES9,0.0083-0.0202; FES10,0.0202-0.0604; FES11,0.4066-0.5101; FES12,0.2424-0.3099;FES13, 0.1860-0.2198; FES14,0.0041-0.0165; and FES15,0-0.0050, respectively. In the German population, a variation in 5 flanking region and nine alleles at FES locus were noted. The allele frequencies were FES8,0.0120; FES10a,0.02317; FES10,0.0407; FES11a,0.0122; FES11,0.4146; FES12a, 0.0041; FES12, 0.2357; FES13, 0.0447; and FES14, 0.0041, respectively. The results of test for Hardy-Weinberg equilibrium showed that the genotype distributions observed in the populations were correspondent with the expected.

CONCLUSION

There is a remarkable difference in the distribution of allele frequencies at FES locus between Chinese and German populations.