Zhang Hong-qin, Wu Shu-zhen, Bi Yun-tian
Molecular Biology Research Centre of Wenzhou Medical College, Wenzhou 325027, China.
Fa Yi Xue Za Zhi. 2004;20(3):160-1.
To study genetic polymorphism of D7S2846, D19S400 and D18S535 in Han population in Wenzhou.
DNA was extracted with chelex-100 method from EDTA-blood samples of 194 unrelated individuals in Wenzhou and amplified with PCR technique. The PCR products were analyzed by PAG vertical electrophoresis and silver-stain.
6 alleles and 15 genotypes of D7S2846, 10 alleles and 36 genotypes of D19S400, 8 alleles and 26 genotypes of D18S535 was observed. The heterozygosities of the three loci are 0.644, 0.724 and 0.772; The power of discriminating are 0.854, 0.940 and 0.938.
The heterozygosities of the three loci are high and the frequencies was in accordance with Hardy-Weinberg equilibrium (P>0.05), so the three loci can be used in forensic medicine and in other genetic researches.
研究温州汉族人群中D7S2846、D19S400和D18S535基因座的遗传多态性。
采用chelex-100法从温州194名无关个体的EDTA抗凝血样中提取DNA，用PCR技术进行扩增。PCR产物经聚丙烯酰胺凝胶垂直电泳和银染分析。
观察到D7S2846有6个等位基因和15种基因型，D19S400有10个等位基因和36种基因型，D18S535有8个等位基因和26种基因型。这三个基因座的杂合度分别为0.644、0.724和0.772；鉴别力分别为0.854、0.940和0.938。
这三个基因座杂合度高，基因频率符合Hardy-Weinberg平衡（P>0.05），可用于法医学及其他遗传学研究。
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