[珠蛋白链的毛细管自由区电泳]
[Capillary free zone electrophoresis of globin chains].
作者信息
Liao Y, Qian X, Xu X
机构信息
Department of Pediatrics, Nanfang Hospital, First Military Medical University, Guangzhou, 510515 P.R. China.
出版信息
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 1999 Jun;16(3):180-4.
OBJECTIVE
To introduce a new technique for rapid separation of globin chains from clinical samples.
METHODS
The authors used the uncoated capillary and the electrophoresis buffer which contained 100mmol/L NaH2PO4 +7mmol/L urea +1% Triton X-100 +1% 2-mercaptoethanol +0.25% PEG-600. Capillary free zone electrophoresis was employed in analyzing the hemoglobin-water solutions which were made from HbA control and 74 clinical blood samples. The globin chains were separated from the samples.
RESULTS
IN 40 minutes, five globin chains: alpha, beta, Ggamma, delta, Agamma were separated clearly by capillary free zone electrophoresis using nanolitres of sample. In electrophoresis figure the baselines were stable and the peaks were clear. The time coefficient of variation (CV) was under 3%, and the area CV below 4%. Also, The authors measured the relative concentration of globin chains from 60 normal cases and 14 thalassemia patients.
CONCLUSION
The capillary free zone electrophoresis for the separation of globin chains from clinical samples has been successfully achieved. It is a rapid, simple and sensitive technique suitable for rapid diagnosis of hemoglobinopathies.
目的
介绍一种从临床样本中快速分离珠蛋白链的新技术。
方法
作者使用未涂层毛细管和含有100mmol/L磷酸二氢钠+7mmol/L尿素+1% Triton X - 100 +1% 2 - 巯基乙醇+0.25%聚乙二醇-600的电泳缓冲液。采用毛细管区带电泳分析由HbA对照品和74份临床血液样本制成的血红蛋白水溶液，从样本中分离珠蛋白链。
结果
40分钟内，使用纳升级样本通过毛细管区带电泳清晰分离出α、β、Gγ、δ、Aγ五条珠蛋白链。电泳图谱基线稳定，峰形清晰。时间变异系数（CV）低于3%，面积CV低于4%。作者还测定了60例正常人和14例地中海贫血患者珠蛋白链的相对浓度。
结论
已成功实现从临床样本中分离珠蛋白链的毛细管区带电泳。这是一种快速、简单且灵敏的技术，适用于血红蛋白病的快速诊断。
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