Gulbis B, Kagambega F, Tshilolo L, Vertongen F
Department of Clinical Chemistry, Hôpital Erasme, Brussels, Belgium.
Ann Clin Biochem. 1998 May;35 ( Pt 3):415-7. doi: 10.1177/000456329803500311.
To improve the resolution and rapidity of globin chains separation, we have modified the basic technique of globin chain electrophoresis in urea-acetic acid-Triton X-100. Haemolysates from anticoagulated cord or adult blood samples were submitted to urea-acetic acid-Triton X-100 polyacrylamide gel electrophoresis using a 15% polyacrylamide gel cast in a mini slab cell which allows a rapid analysis of globin chains samples. After staining proteins with Coomassie brilliant blue R-250, the relative amounts of globin chains were determined by scanning. This new procedure has allowed us to obtain a better separation of the normal and abnormal globin chains than described previously. All the normal globin chains, i.e. A gamma, G gamma, delta, beta and alpha, are well separated by this modified technique. Semi-quantification of the G gamma/A gamma ratio has been performed. This simple and rapid method is also suitable for the global identification of the globin chain involved in the most common abnormal haemoglobin variants, except beta-S.
为提高珠蛋白链分离的分辨率和速度，我们改进了尿素 - 乙酸 - 曲拉通X - 100中珠蛋白链电泳的基本技术。将抗凝脐带血或成人血样的溶血产物进行尿素 - 乙酸 - 曲拉通X - 100聚丙烯酰胺凝胶电泳，使用在迷你平板电泳槽中灌制的15%聚丙烯酰胺凝胶，可快速分析珠蛋白链样本。用考马斯亮蓝R - 250对蛋白质染色后，通过扫描测定珠蛋白链的相对含量。这一新方法使我们能够比之前描述的方法更好地分离正常和异常珠蛋白链。通过这种改进技术，所有正常珠蛋白链，即Aγ、Gγ、δ、β和α，都能得到很好的分离。已对Gγ/Aγ比值进行了半定量。这种简单快速的方法也适用于除β - S外最常见异常血红蛋白变体中涉及的珠蛋白链的全面鉴定。
