Yoshida M, Fukunaga K, Tsuchita H, Yasumoto K
Department of Biotechnology, Faculty of Engineering, Kansai University, Osaka, Japan.
J Nutr Sci Vitaminol (Tokyo). 1999 Jan;45(1):119-28. doi: 10.3177/jnsv.45.119.
The bioavailability of selenium (Se) in high-Se yeast (SeY) was evaluated by measuring tissue Se accumulation and glutathione peroxidase (GSHPx) activity. For 4 weeks, 4-week-old male Wistar rats were fed a Torula yeast-based Se-deficient diet (basal diet) or a diet supplemented with a graded level (0.04, 0.08, 0.16, and 0.32 microgram/g) of Se as either sodium selenite or SeY, which was obtained from two different sources. Se supplementation did not influence growth, hematological values, or serum biochemical tests. Se contents and GSHPx activities in the liver, serum, and erythrocytes increased gradually with increases of the supplemented Se. At lower Se levels (0.04 and 0.08 microgram/g), selenite produced higher Se deposition and higher GSHPx activities than SeY did, but at a higher Se level (0.32 microgram/g), SeY showed higher measures. Strong correlations were detected between the supplementary Se levels and the tissue Se contents or GSHPX activities when the regression was fitted to this equation: R-Rb = m log X + k, where R represented tissue Se content or GSHPx activity in rats fed the diet supplemented with Se at X level, Rb corresponding mean value in rats fed the basal diet, m slope, and k constant. The bioavailability of Se in SeY, as assessed by slope ratio analysis using selenite as a reference Se, was 135% to 165% in the tissue Se content and 105% to 197% in the GSHPx activities. These results indicate that Se in SeY is more bioavailable than selenite Se, and therefore it is the preferred form for supplementation.
通过测量组织中硒（Se）的积累和谷胱甘肽过氧化物酶（GSHPx）活性，评估了高硒酵母（SeY）中硒的生物利用度。将4周龄雄性Wistar大鼠喂食基于球拟酵母的缺硒日粮（基础日粮）或补充有不同梯度水平（0.04、0.08、0.16和0.32微克/克）硒的日粮，硒源为亚硒酸钠或来自两个不同来源的SeY。补充硒对生长、血液学指标或血清生化检测没有影响。肝脏、血清和红细胞中的硒含量和GSHPx活性随着补充硒的增加而逐渐增加。在较低硒水平（0.04和0.08微克/克）时，亚硒酸盐比SeY产生更高的硒沉积和更高的GSHPx活性，但在较高硒水平（0.32微克/克）时，SeY表现出更高的测量值。当将回归拟合到该方程时：R - Rb = m log X + k，其中R代表喂食含X水平硒日粮的大鼠的组织硒含量或GSHPx活性，Rb代表喂食基础日粮的大鼠的相应平均值，m为斜率，k为常数，检测到补充硒水平与组织硒含量或GSHPX活性之间存在强相关性。以亚硒酸盐作为参考硒，通过斜率比分析评估，SeY中硒的生物利用度在组织硒含量方面为135%至165%，在GSHPx活性方面为105%至197%。这些结果表明，SeY中的硒比亚硒酸盐硒具有更高的生物利用度，因此它是补充的首选形式。
