Liu Guoqing, Cao Sumei, Huang Liang, Lin Xuanxu, Sun Zheng, Lin Gang, Zhang Liyang, Lu Lin, Luo Xugang, Liao Xiudong
Mineral Nutrition Research Division, State Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China.
Department of Animal Science, Hebei Normal University of Science and Technology, Qinhuangdao, China.
Front Vet Sci. 2025 Jan 17;11:1542557. doi: 10.3389/fvets.2024.1542557. eCollection 2024.
Selenium (Se) is an essential trace element for humans and animals. Development and application of new forms of Se sources with lower toxicity and higher bioavailability has been attracting more attention. However, the bioavailabilities of Se from several new Se sources for broilers remain unclear. Therefore, the aim of this study was to assess the relative bioavailabilities of Se from Se yeast (SY), selenomethionine (SM), hydroxyl-selenomethionine (SO) and nano-Se (NS) relative to sodium selenite (SS) for broilers fed a conventional corn-soybean meal diet. A total of 576 one-day-old Arbor Acres commercial male broilers were randomly assigned to 16 treatments with 6 replicate cages per treatment in a completely randomized design involving a 5 (Se sources: SY, SM, SO, NS and SS) × 3 (added Se levels: 0.15, 0.30 and 0.45 mg Se/kg) factorial design of treatments plus 1 (a Se-unsupplemented control) for 21 d. The relative bioavailabilities of Se sources were estimated based on plasma or tissue Se concentrations as well as selenoprotein mRNA expressions and activities in broilers. The results showed that the Se concentrations and glutathione peroxidase (GPX) activities in plasma, liver, breast muscle, pancreas and kidney as well as Se concentration in erythrocytes of broilers, and and mRNA expressions in pancreas increased linearly ( < 0.03) as added Se level increased. Furthermore, the differences ( < 0.05) among different Se sources were detected for the Se concentrations in liver, breast muscle, pancreas and erythrocytes, GPX activities in pancreas and kidney. Based on slope ratios from the multiple linear regressions of the above indices, the Se bioavailabilities of SY, SM, SO, NS relative to SS (100%) were 78 to 367%, 67.8 to 471%, 57 to 372%, and 45 to 92%, respectively. The results from this study indicated that the Se from SM, SY and SO are more available to broilers than the Se from SS in enhancing the Se concentrations in liver, breast muscle, pancreas and erythrocytes and GPX activity in pancreas, and the Se from SM had the highest while the Se from NS had the lowest relative bioavailability.
硒（Se）是人和动物必需的微量元素。开发毒性更低、生物利用度更高的新型硒源并加以应用，一直备受关注。然而，几种新型硒源对肉鸡的生物利用度仍不明确。因此，本研究旨在评估相对于亚硒酸钠（SS），酵母硒（SY）、硒代蛋氨酸（SM）、羟基硒代蛋氨酸（SO）和纳米硒（NS）对饲喂常规玉米 - 豆粕日粮肉鸡的相对生物利用度。总共576只1日龄的爱拔益加商品雄性肉鸡被随机分配到16个处理组，每个处理组有6个重复笼，采用完全随机设计，涉及5（硒源：SY、SM、SO、NS和SS）×3（添加硒水平：0.15、0.30和0.45毫克硒/千克）析因设计处理组加1个（未添加硒的对照组），试验期为21天。根据肉鸡血浆或组织中的硒浓度以及硒蛋白mRNA表达和活性来估计硒源的相对生物利用度。结果表明，随着添加硒水平的增加，肉鸡血浆、肝脏、胸肌、胰腺和肾脏中的硒浓度以及红细胞中的硒浓度，以及胰腺中的谷胱甘肽过氧化物酶（GPX）活性和mRNA表达呈线性增加（P<0.03）。此外，在肝脏、胸肌、胰腺和红细胞中的硒浓度、胰腺和肾脏中的GPX活性方面，不同硒源之间存在差异（P<0.05）。基于上述指标的多元线性回归斜率比，SY、SM、SO、NS相对于SS（100%）的硒生物利用度分别为78%至367%、67.8%至471%、57%至372%和45%至92%。本研究结果表明，在提高肝脏、胸肌、胰腺和红细胞中的硒浓度以及胰腺中的GPX活性方面，SM、SY和SO中的硒对肉鸡的有效性高于SS中的硒，其中SM中的硒相对生物利用度最高，而NS中的硒相对生物利用度最低。
