Sakata N, Yamazaki K, Kogure T
Department of Legal Medicine, St. Marianna University School of Medicine, Kawasaki, Japan.
Zentralbl Bakteriol. 1999 Apr;289(2):217-25. doi: 10.1016/s0934-8840(99)80109-1.
Binding of Candida albicans strain JCM0239 to radiolabelled laminin was studied to identify possible receptors. When two forms of the organism were examined, laminin was found to bind to germinating blastoconidia. There was only weak binding to blastoconidia without germ tubes. Laminin-binding to germinating blastoconidia was inhibited by non-labelled laminin or fibrinogen but not by fibronectin, a fibronectin peptide (Gly-Arg-Gly-Asp-Ser), or a laminin peptide (Tyr-Ile-Gly-Ser-Arg). A cell extract from germinating blastoconidia was analyzed by using SDS-PAGE and Western blotting, and 125I-labelled laminin was shown to bind to a 21 kDa component.
研究了白色念珠菌菌株JCM0239与放射性标记的层粘连蛋白的结合，以鉴定可能的受体。当检查该生物体的两种形式时，发现层粘连蛋白与发芽的芽生孢子结合。与没有芽管的芽生孢子只有微弱的结合。未标记的层粘连蛋白或纤维蛋白原可抑制层粘连蛋白与发芽芽生孢子的结合，但纤连蛋白、纤连蛋白肽（甘氨酸-精氨酸-甘氨酸-天冬氨酸-丝氨酸）或层粘连蛋白肽（酪氨酸-异亮氨酸-甘氨酸-丝氨酸-精氨酸）则不能。通过SDS-PAGE和蛋白质免疫印迹分析发芽芽生孢子的细胞提取物，结果显示125I标记的层粘连蛋白与一个21 kDa的成分结合。
