Glee P M, Masuoka J, Ozier W T, Hazen K C
Department of Pathology, University of Virginia Medical Center, Charlottesville 22908, USA.
J Med Vet Mycol. 1996 Jan-Feb;34(1):57-61. doi: 10.1080/02681219680000091.
Candida albicans has been reported to express only one to three proteins that bind extracellular matrix proteins, such as laminin and fibrinogen. In those reports, cell wall extracts were subjected to various processing steps, such as dialysis and lyophilization, prior to Western blot analysis. Here, we demonstrate that dialysis for only 2 h of cell wall protein extracts results in a substantial loss (40-60%) of protein. With overnight dialysis, the loss was increased further. After 2 h of dialysis, wall extracts contained fewer laminin- and fibronectin-reactive proteins. In addition, the number of wall proteins in the extracts detected by a polyclonal anti-human fibronectin receptor antiserum decreased after dialysis. These results demonstrate that the C. albicans yeast cell wall contains multiple proteins capable of binding laminin and fibronectin and many of these proteins are not functionally detectable following dialysis.
据报道，白色念珠菌仅表达一至三种能结合细胞外基质蛋白（如层粘连蛋白和纤维蛋白原）的蛋白质。在那些报道中，细胞壁提取物在进行蛋白质印迹分析之前要经过各种处理步骤，如透析和冻干。在此，我们证明细胞壁蛋白提取物仅透析2小时就会导致大量蛋白质损失（40%-60%）。过夜透析后，损失进一步增加。透析2小时后，细胞壁提取物中与层粘连蛋白和纤连蛋白反应的蛋白质减少。此外，用多克隆抗人纤连蛋白受体抗血清检测时，透析后提取物中细胞壁蛋白的数量减少。这些结果表明，白色念珠菌酵母细胞壁含有多种能够结合层粘连蛋白和纤连蛋白的蛋白质，并且其中许多蛋白质在透析后无法进行功能检测。
