Brodeur G M, Goldstein M N
Cytobios. 1976;16(62):133-8.
Cells of three established lines of human neuroblastoma and an established line of C1300 mouse neuroblastoma were grown in control medium or in experimental medium containing mouse nerve growth factor (NGF). Cultures were stained histochemically for acetylcholinesterase (AChE) during log growth and at confluency. Human neuroblastoma cells grown in medium containing NGF were morphologically more differentiated and they were stained much more intensely for AChE during both phases of growth than were cells in control cultures. The enzyme was distributed over cell bodies and neurites. Neuroblastoma cells of the mouse line were not stimulated to form neurites by NGF, but they were more intensely stained for acetylcholinesterase than cells grown in control medium. These observations support earlier findings that NGF stimulates differentiation of human and mouse neuroblastoma cells in vitro.
三种已建立的人神经母细胞瘤细胞系和一种已建立的C1300小鼠神经母细胞瘤细胞系在对照培养基或含有小鼠神经生长因子（NGF）的实验培养基中培养。在对数生长期和汇合期，对培养物进行乙酰胆碱酯酶（AChE）的组织化学染色。在含有NGF的培养基中生长的人神经母细胞瘤细胞在形态上更具分化性，并且在两个生长阶段中，它们的AChE染色都比对照培养物中的细胞强烈得多。该酶分布在细胞体和神经突上。小鼠系的神经母细胞瘤细胞未被NGF刺激形成神经突，但它们的乙酰胆碱酯酶染色比在对照培养基中生长的细胞更强。这些观察结果支持了早期的发现，即NGF在体外刺激人和小鼠神经母细胞瘤细胞的分化。
