Berwald-Netter Y, Jeantet C, Vimard C
Natl Cancer Inst Monogr. 1978 May(48):359-61.
Mouse neuroblastoma clone NIE-115 contains two species of acetylcholinesterase (AChE) which sediment at 4S and 11S. The former is predominant in growing cells (70%), whereas the 11S is more abundant in cells with neurites (55--65%). When cell replication is arrested by sodium butyrate, there is an increase in AChE activity but no modification in the proportion of 4S:11S species. Inhibition of protein synthesis by cycloheximide provokes a slow increase in the 11S form and a parallel decrease in 4S. Neurite retraction in presence of colchicine or excess serum does not lead to a concomitant reversion in the high 11S proportion. We postulate that the 11S AChE is formed by a conversion of the 4S and that the shift in the S-value ratio of the enzyme reflects changes in membrane remodeling during neurite extension.
小鼠神经母细胞瘤克隆NIE-115含有两种乙酰胆碱酯酶（AChE），它们的沉降系数分别为4S和11S。前者在生长中的细胞中占主导地位（70%），而11S在有神经突的细胞中更为丰富（55%-65%）。当细胞复制被丁酸钠阻断时，AChE活性增加，但4S:11S种类的比例没有改变。放线菌酮抑制蛋白质合成会导致11S形式缓慢增加，同时4S形式平行减少。在秋水仙碱或过量血清存在的情况下，神经突回缩不会导致高比例11S的同时逆转。我们推测11S AChE是由4S转化形成的，酶的S值比例变化反映了神经突延伸过程中膜重塑的变化。
