亚硫酸氢盐测序方案可显示5-甲基胞嘧啶和N4-甲基胞嘧啶。 - Suppr

Suppr

超能文献

Bisulfite sequencing protocol displays both 5-methylcytosine and N4-methylcytosine.

作者信息

Vilkaitis G, Klimasauskas S

机构信息

Laboratory of Biological DNA Modification, Institute of Biotechnology, Graiciūno 8, Vilnius, LT-2028, Lithuania.

出版信息

Anal Biochem. 1999 Jun 15;271(1):116-9. doi: 10.1006/abio.1999.4116.

DOI:10.1006/abio.1999.4116

PMID:10361019

Abstract

摘要

相似文献

Bisulfite sequencing protocol displays both 5-methylcytosine and N4-methylcytosine.

Anal Biochem. 1999 Jun 15;271(1):116-9. doi: 10.1006/abio.1999.4116.

Novel procedure for the detection of 5-methylcytosine.

Acta Microbiol Pol. 1998;47(4):327-34.

A bisulfite method of 5-methylcytosine mapping that minimizes template degradation.

Anal Biochem. 1995 Mar 20;226(1):161-6. doi: 10.1006/abio.1995.1204.

The bisulfite genomic sequencing used in the analysis of epigenetic states, a technique in the emerging environmental genotoxicology research.

Mutat Res. 2008 Jul-Aug;659(1-2):77-82. doi: 10.1016/j.mrrev.2008.04.003. Epub 2008 May 14.

DNA methylation analysis by bisulfite conversion, cloning, and sequencing of individual clones.

Methods Mol Biol. 2009;507:177-87. doi: 10.1007/978-1-59745-522-0_14.

Sequencing 5-methylcytosine residues in genomic DNA.

Bioessays. 1994 Jun;16(6):431-6. doi: 10.1002/bies.950160612.

Use of a single sequencing termination reaction to distinguish between cytosine and 5-methylcytosine in bisulfite-modified DNA.

Biotechniques. 1997 May;22(5):850-4. doi: 10.2144/97225bm14.

Does urea promote the bisulfite-mediated deamination of cytosine in DNA? Investigation aiming at speeding-up the procedure for DNA methylation analysis.

Nucleic Acids Symp Ser (Oxf). 2006(50):69-70. doi: 10.1093/nass/nrl034.

Bisulfite genomic sequencing: systematic investigation of critical experimental parameters.

Nucleic Acids Res. 2001 Jul 1;29(13):E65-5. doi: 10.1093/nar/29.13.e65.

Cytosines adjacent to methylated CpG sites can be partially resistant to conversion in genomic bisulfite sequencing leading to methylation artifacts.

Anal Biochem. 1998 Nov 1;264(1):129-32. doi: 10.1006/abio.1998.2833.

引用本文的文献

Shaping eukaryotic epigenetic systems by horizontal gene transfer.

Bioessays. 2023 Jul;45(7):e2200232. doi: 10.1002/bies.202200232.

Genome-wide mapping of -methylcytosine at single-base resolution by APOBEC3A-mediated deamination sequencing.

Chem Sci. 2022 Aug 11;13(34):9960-9972. doi: 10.1039/d2sc02446b. eCollection 2022 Aug 31.

Rapid identification of methylase specificity (RIMS-seq) jointly identifies methylated motifs and generates shotgun sequencing of bacterial genomes.

Nucleic Acids Res. 2021 Nov 8;49(19):e113. doi: 10.1093/nar/gkab705.

METTL15 introduces N4-methylcytidine into human mitochondrial 12S rRNA and is required for mitoribosome biogenesis.

Nucleic Acids Res. 2019 Nov 4;47(19):10267-10281. doi: 10.1093/nar/gkz735.

Divergent methylation of CRISPR repeats and cas genes in a subtype I-D CRISPR-Cas-system.

BMC Microbiol. 2019 Jul 1;19(1):147. doi: 10.1186/s12866-019-1526-3.

Deciphering bacterial epigenomes using modern sequencing technologies.

Nat Rev Genet. 2019 Mar;20(3):157-172. doi: 10.1038/s41576-018-0081-3.

Transgenerational Epigenetic Inheritance Under Environmental Stress by Genome-Wide DNA Methylation Profiling in Cyanobacterium.

Front Microbiol. 2018 Jul 4;9:1479. doi: 10.3389/fmicb.2018.01479. eCollection 2018.

Selective recognition of 4-methylcytosine in DNA by engineered transcription-activator-like effectors.

Philos Trans R Soc Lond B Biol Sci. 2018 Jun 5;373(1748). doi: 10.1098/rstb.2017.0078.

Identification of the DNA methyltransferases establishing the methylome of the cyanobacterium Synechocystis sp. PCC 6803.

DNA Res. 2018 Aug 1;25(4):343-352. doi: 10.1093/dnares/dsy006.

Base-resolution detection of N4-methylcytosine in genomic DNA using 4mC-Tet-assisted-bisulfite- sequencing.

Nucleic Acids Res. 2015 Dec 2;43(21):e148. doi: 10.1093/nar/gkv738. Epub 2015 Jul 15.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型，支持多种主流文档格式。

立即体验