对基因组DNA中的5-甲基胞嘧啶残基进行测序。
Sequencing 5-methylcytosine residues in genomic DNA.
作者信息
Grigg G, Clark S
机构信息
Peptide Technology Ltd, Sydney, NSW, Australia.
出版信息
Bioessays. 1994 Jun;16(6):431-6. doi: 10.1002/bies.950160612.
PMID:8080433
Abstract
To analyse the biological role of 5-methylation of cytosine residues in DNA requires precise and efficient methods for detecting individual 5-methylcytosines (5-MeCs) in genomic DNA. The methods developed over the past decade rely on either differential enzymatic or chemical cleavage of DNA, or more recently on differential sensitivity to chemical conversion of one base to another. The most commonly used methods for studying the methylation profile of DNA, including the bisulphite base-conversion method, are reviewed.
摘要
要分析DNA中胞嘧啶残基5-甲基化的生物学作用，需要精确且高效的方法来检测基因组DNA中的单个5-甲基胞嘧啶（5-MeC）。过去十年中开发的方法要么依赖于DNA的差异酶切或化学切割，要么更近期地依赖于一种碱基向另一种碱基化学转化的差异敏感性。本文综述了研究DNA甲基化谱最常用的方法，包括亚硫酸氢盐碱基转化法。
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