Raizis A M, Schmitt F, Jost J P
Friedrich Miescher Institut, Basel, Switzerland.
Anal Biochem. 1995 Mar 20;226(1):161-6. doi: 10.1006/abio.1995.1204.
The bisulfite method is a highly sensitive approach to 5-methylcytosine mapping that utilizes the capability of the polymerase chain reaction to exponentially amplify DNA. We have observed that the bisulfite reaction results in a significant level of template degradation due to DNA depurination. Furthermore, our data suggest that the DNA fragmentation which occurs limits the sensitivity of the method. We describe a simple solution to limit degradation of the DNA template.
亚硫酸氢盐法是一种用于5-甲基胞嘧啶图谱绘制的高灵敏度方法，它利用聚合酶链反应对DNA进行指数级扩增的能力。我们观察到，由于DNA脱嘌呤作用，亚硫酸氢盐反应会导致显著水平的模板降解。此外，我们的数据表明，发生的DNA片段化限制了该方法的灵敏度。我们描述了一种限制DNA模板降解的简单解决方案。
