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[获取用于研究细胞相互作用在早期发育中作用的金线鱼嵌合胚胎]

[Obtaining chimeric embryos of groundling for study of the role of cellular interactions in early development].

作者信息

Bozhkova V P, Rott N N

出版信息

Ontogenez. 1976;7(3):309-15.

PMID:1036112
Abstract

A methos of production of chimaeric embryos of the loach by parabiosis of whole embryos and a whole embryo and an isolated blastodisc is proposed. The parabiosis was shown to be possible between the embryos of both the same and different ages during cleavage and blastulation. The relation between the frequency of parabiosis and the age of embryos and temperature was studied. An electric coupling is established between the cells of parabionts, similar by its value to that between the cells of each parabiont. The interrelations of cells of chimaeric embryos are considered as a model for studying the role of cell interactions during embryogenesis.

摘要

提出了一种通过全胚胎与全胚胎、全胚胎与分离胚盘联体共生来生产泥鳅嵌合体胚胎的方法。结果表明,在卵裂和囊胚形成过程中,相同年龄和不同年龄的胚胎之间都可以进行联体共生。研究了联体共生频率与胚胎年龄和温度之间的关系。联体共生体的细胞之间建立了电耦合,其值与每个联体共生体细胞之间的电耦合相似。嵌合体胚胎细胞的相互关系被视为研究胚胎发生过程中细胞相互作用作用的模型。

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