Gimeno A L, Goldraij A, Gimeno M F, Santillan de Torres R
Reproduccion. 1976 Jan-Jun;3(1-2):5-14.
The stability with time of the spontaneous as well as the oxytocin-triggered functional activity of uterine borns isolated from induced estrus rats and immersed in a medium with lactate as the substrate was not affected by the presence of oxomate (an inhibitor of lactate dehydrogenase) at 10 or 20mM. On the contrary, the oxytocin-driven and the spontaneous motility of preparations obtained from 15 days castrated rats diminished significantly following in addition of the enzyme inhibitor. Furthermore, uterine borns from ovariectomized animals injected with 17-beta estradiol, regain the lack of functional sensivity towards oxamate. In addition, determinations of pyruvate levels in homogenates of uterine tissue, previously suspended in lactate medium (as in the functional experiments), demonstrate to be significantly reduced, following oxamate. Several possible reasons, attempting to explain these findings, are discussed.
从诱导发情大鼠分离并浸入以乳酸为底物的培养基中的子宫肌条，其自发以及催产素触发的功能活性随时间的稳定性不受10或20mM草氨酸盐（乳酸脱氢酶抑制剂）的影响。相反，添加酶抑制剂后，从15天去势大鼠获得的制剂的催产素驱动和自发运动性显著降低。此外，注射17-β雌二醇的去卵巢动物的子宫肌条恢复了对草氨酸盐缺乏功能敏感性的状态。另外，先前悬浮于乳酸培养基中的子宫组织匀浆（如在功能实验中）中的丙酮酸水平测定表明，加入草氨酸盐后丙酮酸水平显著降低。文中讨论了试图解释这些发现的几个可能原因。
