Kawana K, Yoshikawa H, Taketani Y, Yoshiike K, Kanda T
Division of Molecular Genetics, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo 162-8640, Japan.
J Virol. 1999 Jul;73(7):6188-90. doi: 10.1128/JVI.73.7.6188-6190.1999.
Studies of virus neutralization by antibody are a prerequisite for development of a prophylactic vaccine strategy against human papillomaviruses (HPVs). Using HPV16 and -6 pseudovirions capable of inducing beta-galactosidase in infected monkey COS-1 cells, we examined the neutralizing activity of mouse monoclonal antibodies (MAbs) that recognize surface epitopes in HPV16 minor capsid protein L2. Two MAbs binding to a synthetic peptide with the HPV16 L2 sequence of amino acids (aa) 108 to 120 were found to inhibit pseudoinfections with HPV16 as well as HPV6. Antisera raised by immunizing BALB/c mice with the synthetic peptide had a cross-neutralizing activity similar to that of the MAb. The data indicate that HPV16 and -6 have a common cross-neutralization epitope (located within aa 108 to 120 of L2 in HPV16), suggesting that this epitope may be shared by other genital HPVs.
通过抗体进行病毒中和研究是开发针对人乳头瘤病毒（HPV）预防性疫苗策略的先决条件。利用能够在感染的猴COS-1细胞中诱导β-半乳糖苷酶的HPV16和-6假病毒颗粒，我们检测了识别HPV16次要衣壳蛋白L2表面表位的小鼠单克隆抗体（MAb）的中和活性。发现两种与含有HPV16 L2氨基酸（aa）序列108至120的合成肽结合的单克隆抗体能够抑制HPV16以及HPV6的假感染。用该合成肽免疫BALB/c小鼠产生的抗血清具有与单克隆抗体相似的交叉中和活性。数据表明HPV16和-6具有共同的交叉中和表位（位于HPV16的L2的aa 108至120内），这表明该表位可能为其他生殖道HPV所共有。
