Herrmann J E, Chen S C, Jones D H, Tinsley-Bown A, Fynan E F, Greenberg H B, Farrar G H
Division of Infectious Diseases and Immunology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA.
Virology. 1999 Jun 20;259(1):148-53. doi: 10.1006/viro.1999.9751.
Protective immune responses in mice were obtained after oral immunization with rotavirus DNA vaccines encapsulated in poly(lactide-co-glycolide) (PLG) microparticles. The DNA vaccines used encoded outer capsid proteins VP4 and VP7; proteins that are the basis for rotavirus serotyping and the generation of virus neutralizing antibodies. One dose of vaccine was given to BALB/c mice by oral gavage (75 microg DNA/mouse). Rotavirus-specific serum antibodies and intestinal IgA antibodies were detectable by 6 weeks postimmunization. After challenge with homologous murine rotavirus at 12 weeks postimmunization, fecal rotavirus antigen was reduced significantly in immunized mice compared with controls. Protective immunity also was generated by oral delivery of unencapsulated VP 7 DNA vaccine but to a lesser degree. These results demonstrate that the oral route is effective for generating protective immune responses with rotavirus DNA vaccines targeting neutralization antigens.
用包裹在聚（丙交酯-共-乙交酯）（PLG）微粒中的轮状病毒DNA疫苗对小鼠进行口服免疫后，获得了保护性免疫反应。所使用的DNA疫苗编码外衣壳蛋白VP4和VP7；这些蛋白是轮状病毒血清分型和产生病毒中和抗体的基础。通过口服灌胃给BALB/c小鼠一剂疫苗（75微克DNA/小鼠）。免疫后6周可检测到轮状病毒特异性血清抗体和肠道IgA抗体。在免疫后12周用同源鼠轮状病毒攻击后，与对照组相比，免疫小鼠的粪便轮状病毒抗原显著减少。口服未包裹的VP7 DNA疫苗也能产生保护性免疫，但程度较小。这些结果表明，口服途径对于用靶向中和抗原的轮状病毒DNA疫苗产生保护性免疫反应是有效的。
