Herrmann J E, Chen S C, Fynan E F, Santoro J C, Greenberg H B, Robinson H L
Division of Infectious Diseases and Immunology, University of Massachusetts Medical School, Worcester, USA.
Arch Virol Suppl. 1996;12:207-15. doi: 10.1007/978-3-7091-6553-9_22.
Plasmid DNA vaccines encoding for murine rotaviral proteins VP4, VP6, and VP7 were tested in adult BALB/c mice for their ability to induce immune responses and provide protection against rotavirus challenge. Serum antibodies were measured by virus neutralization and by ELISA. Cellular immunity was assessed by measuring cytotoxic T cell (CTL) responses. The vaccines were administered by inoculation into cells of the epidermis with an Accell gene gun (Auragen, Inc., Middleton, WI, USA). Each of the three vaccines elicited rotavirus-specific serum antibodies as measured by ELISA. Virus neutralizing antibodies were detected in mice receiving DNA vaccines encoding for VP4 and VP7, but not in those which received the plasmid encoding for VP6. Vaccines encoding for VP4, VP6, or VP7 generated virus-specific CTL responses in recipient mice. Efficacy of the vaccines was determined by challenge with homotypic rotaviruses. Each of the three vaccines was effective in protecting mice against infection after rotavirus (100 ID50) challenge. Significant reductions (p < 0.0002, analysis of variance) in viral excretion measured over a 9 day period were seen in mice receiving the DNA vaccines compared with mice that received control plasmids.
对编码鼠轮状病毒蛋白VP4、VP6和VP7的质粒DNA疫苗在成年BALB/c小鼠中进行了测试，以评估其诱导免疫反应及提供针对轮状病毒攻击的保护能力。通过病毒中和试验和酶联免疫吸附测定法检测血清抗体。通过测量细胞毒性T细胞（CTL）反应评估细胞免疫。使用Accell基因枪（美国威斯康星州米德尔顿市的Auragen公司）将疫苗接种到表皮细胞中。通过酶联免疫吸附测定法测定，三种疫苗均能诱导出轮状病毒特异性血清抗体。在接受编码VP4和VP7的DNA疫苗的小鼠中检测到病毒中和抗体，但在接受编码VP6的质粒的小鼠中未检测到。编码VP4、VP6或VP7的疫苗在受体小鼠中产生了病毒特异性CTL反应。通过用同型轮状病毒攻击来确定疫苗的效力。三种疫苗均能有效保护小鼠免受轮状病毒（100个半数感染剂量）攻击后的感染。与接受对照质粒的小鼠相比，接受DNA疫苗的小鼠在9天内测量的病毒排泄量显著降低（方差分析，p < 0.0002）。
