Musiani M, Pasini P, Zerbini M, Gentilomi G, Roda A, Gallinella G, Manaresi E, Venturoli S
Division of Microbiology, Department of Clinical and Experimental Medicine, University of Bologna, Bologna, Italy.
J Clin Microbiol. 1999 Jul;37(7):2326-9. doi: 10.1128/JCM.37.7.2326-2329.1999.
Parvovirus B19 can be transmitted transplacentally from the infected mother to the fetus during pregnancy, and hydrops fetalis, abortion, or stillbirth can result. In our study we explored the use of chemiluminescence in situ hybridization to detect B19 DNA on cord blood cells, amniotic fluid cells, and pleuric fluid cells from several cases of hydrops fetalis. B19 DNA was detected by using digoxigenin-labeled probes immunoenzymatically visualized with the chemiluminescent adamantil-1,2-dioxetane phenyl phosphate substrate for alkaline phosphatase. The luminescent signal emitted from the hybridized probes was detected, analyzed, and measured with a high-performance, low-light-level imaging luminograph connected to an optical microscope and to a personal computer for the quantification and localization of the chemiluminescent emission inside individual cells.
细小病毒B19在孕期可经胎盘从感染的母亲传播给胎儿，可导致胎儿水肿、流产或死产。在我们的研究中，我们探索了使用化学发光原位杂交技术，以检测数例胎儿水肿病例的脐血细胞、羊水细胞和胸水细胞中的B19 DNA。采用地高辛标记的探针，通过免疫酶法，使用碱性磷酸酶的化学发光金刚烷-1,2-二氧杂环丁烷苯基磷酸底物进行可视化检测B19 DNA。使用连接到光学显微镜和个人计算机的高性能、低光水平成像发光仪，检测、分析和测量杂交探针发出的发光信号，以对单个细胞内的化学发光发射进行定量和定位。
