肾素-血管紧张素系统基因多态性与原发性高血压

[Genetic polymorphisms of the renin-angiotensin system and essential hypertension].

作者信息

Fernández-Llama P, Poch E, Oriola J, Botey A, de la Sierra A, Revert L, Rivera F, Darnell A

机构信息

Servicio de Nefrología, Hospital Clínic i Provincial, Barcelona.

出版信息

Med Clin (Barc). 1999 May 1;112(15):561-4.

PMID:10365380

Abstract

BACKGROUND

The renin-angiotensin system (RAS) plays an important role in blood pressure (BP) regulation. A number of RAS polymorphisms have been linked to essential hypertension (EH), but there is uncertainty about this association in other studies. We examined whether the insertion/deletion (I/D) polymorphism of the angiotensin converting enzyme (ACE) gene, and the M235T and T174M polymorphisms of the angiotensinogen (AGT) gene are associated with EH in a Spanish sample of hypertensive patients.

MATERIAL AND METHODS

We studied 75 patients with EH (BP > 160/100 mmHg), aged 55 (8.5) years, 30 males, systolic BP (SBP) 182 +/- (22.1) mmHg, diastolic BP (DBP) 109 +/- (9.9) mmHg (mean [SD]) and a strong family history of the disease. As a control group, 75 healthy subjects with no family history of cardiovascular disease were studied. The polymorphisms were determined by PCR amplification of genomic DNA, followed by enzyme digestion for the AGT gene polymorphisms.

RESULTS

The genotype distribution and the frequencies of the alleles of the three RAS polymorphisms were similar in hypertensive and control subjects. In addition, we did not find any compound effect of the I/D ACE gene and M235T AGT gene polymorphisms on BP levels in hypertensive and control subjects.

CONCLUSIONS

In this sample, the contribution of the ACE I/D polymorphism and the AGT M235T and T174M polymorphisms in the development of EH seems to be less important than previously estimated.

摘要

背景

肾素 - 血管紧张素系统（RAS）在血压（BP）调节中起重要作用。许多RAS基因多态性与原发性高血压（EH）有关，但其他研究中这种关联存在不确定性。我们在一组西班牙高血压患者样本中研究了血管紧张素转换酶（ACE）基因的插入/缺失（I/D）多态性以及血管紧张素原（AGT）基因的M235T和T174M多态性是否与EH相关。

材料与方法

我们研究了75例EH患者（血压> 160/100 mmHg），年龄55（8.5）岁，男性30例，收缩压（SBP）182±（22.1）mmHg，舒张压（DBP）109±（9.9）mmHg（均值[标准差]），且有强烈的家族病史。作为对照组，研究了75例无心血管疾病家族史的健康受试者。通过基因组DNA的PCR扩增确定多态性，随后对AGT基因多态性进行酶切。