突尼斯2型糖尿病患者肾素-血管紧张素系统（RAS）基因多态性与高血压之间无关联。
Lack of association between renin-angiotensin system (RAS) polymorphisms and hypertension in Tunisian type 2 diabetics.
作者信息
Arfa Imen, Nouira Sonia, Abid Abdelmajid, Bouafif-Ben Alaya Nissaf, Zorgati Mohamed Majdi, Malouche Dhafer, Manai Imen, Ben Rayana Mohamed Chiheb, Blousa-Chabchoub Samira, Ben Romdhane Habiba, Ben Ammar Mohammed Slim, Ben Salah Afif, Abdelhak Sonia
机构信息
Molecular Investigation of Genetic Orphan Diseases Research Unit, Institut Pasteur de Tunis, Tunisia.
出版信息
Tunis Med. 2010 Jan;88(1):38-41.
BACKGROUND
The genes encoding renin-angiotensin system (RAS) components are potent candidate genes in both hypertension and diabetes namely ACE encoding the angiotensin converting enzyme and AGT encoding angiotensinogen. It has been suggested that the insertion/deletion (I/D) polymorphism in intron 16 of ACE gene is associated with ACE levels, and M235T gene polymorphism is associated with plasma AGT levels.
AIM
We examined in this report the association between ACE I/D and AGT M235T polymorphisms with hypertension status in Tunisian type 2 diabetic subjects.
METHODS
Thirty nine hypertensive and 22 normotensive type 2 diabetic Tunisian patients were recruited for this study. The I/D polymorphism of ACE gene was analysed with nested PCR in order to avoid mistyping heterozygous individuals and the M235T polymorphism of AGT gene was analysed using PCR and allele specific restriction.
RESULTS
The distribution of DD, ID and II genotypes did not significantly differ between type 2 diabetic patients with or without hypertension (DD: 49%; ID: 41%; II: 10% vs DD: 36%; ID: 55%; II: 9%, respectively) (chi2=1.06, p=0.58). There was also no significant statistical difference between these two groups for the M235T polymorphism (TT: 20%; MT: 54%; MM: 26% vs TT: 27%; MT: 41%; MM: 32%, respectively) (chi2=0.95, p=0.62).
CONCLUSION
RAS polymorphisms do not seem to play a role in the development of hypertension in the studied Tunisian type 2 diabetic subjects.
背景
编码肾素 - 血管紧张素系统（RAS）组分的基因是高血压和糖尿病的重要候选基因，即编码血管紧张素转换酶的ACE基因和编码血管紧张素原的AGT基因。有人提出，ACE基因第16内含子中的插入/缺失（I/D）多态性与ACE水平相关，而M235T基因多态性与血浆AGT水平相关。
目的
在本报告中，我们研究了突尼斯2型糖尿病患者中ACE I/D和AGT M235T多态性与高血压状态之间的关联。
方法
本研究招募了39名高血压和22名血压正常的突尼斯2型糖尿病患者。为避免对杂合子个体进行错误分型，采用巢式PCR分析ACE基因的I/D多态性，并使用PCR和等位基因特异性限制酶分析AGT基因的M235T多态性。
结果
2型糖尿病高血压患者与非高血压患者之间的DD、ID和II基因型分布无显著差异（DD分别为49%和36%；ID分别为41%和55%；II分别为10%和9%）（χ2 = 1.06，p = 0.58）。这两组之间的M235T多态性也无显著统计学差异（TT分别为20%和27%；MT分别为54%和 41%；MM分别为26%和32%）（χ2 = 0.95，p = 0.62）。
结论
在所研究的突尼斯2型糖尿病患者中，RAS多态性似乎在高血压的发生中不起作用。
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