Kennedy P G, Grinfeld E, Gow J W
Institute of Neurological Sciences, Southern General Hospital NHS Trust, Glasgow, G51 4TF, Scotland, United Kingdom.
Virology. 1999 Jun 5;258(2):451-4. doi: 10.1006/viro.1999.9745.
To understand further the molecular events underlying the process of Varicella-zoster virus (VZV) latency in human ganglionic tissues, in situ hybridisation (ISH) for VZV RNA and DNA, and PCR in situ amplification for VZV DNA were used in human dorsal root ganglia from 12 individuals (3 normal and 9 who had died with AIDS). The results showed that (a) two separate regions of the VZV genome, represented by genes 4 and 40, were detected in neurons in two normal and three AIDS ganglia, (b) evidence of transcription of VZV genes 4, 21, 29, and 63 was found in normal and AIDS cases, and (c) VZV DNA and RNA for the same gene (gene 29) was detected in neurons in serial tissue sections in three cases. Thus more than one region of the VZV genome is present in neurons during VZV ganglionic latency, and the presence of both a VZV gene and its corresponding RNA transcript can be shown to occur in the same localised region of DRG tissue.
为了进一步了解水痘带状疱疹病毒（VZV）在人类神经节组织中潜伏过程的分子机制，对12名个体（3名正常人及9名死于艾滋病者）的人背根神经节进行了VZV RNA和DNA的原位杂交（ISH）以及VZV DNA的PCR原位扩增。结果显示：（a）在2名正常人和3名艾滋病患者的神经节神经元中，检测到了由基因4和40代表的VZV基因组的两个不同区域；（b）在正常人和艾滋病患者中均发现了VZV基因4、21、29和63转录的证据；（c）在3例连续组织切片的神经元中检测到了同一基因（基因29）的VZV DNA和RNA。因此，在VZV神经节潜伏期间，VZV基因组的多个区域存在于神经元中，并且可以证明VZV基因及其相应的RNA转录本存在于DRG组织的同一局部区域。
