Casterline J L, Oles C J, Ku Y
U.S. Food and Drug Administration, Center for Food Safety and Applied Nutrition, Washington, DC 20204, USA.
J AOAC Int. 1999 May-Jun;82(3):759-65.
A separation scheme for the determination of sugars and starch in processed food was developed. It is based on AOAC Method 985.29 for total dietary fiber with these modifications: carbohydrate starches are separated into soluble and insoluble fractions before they are hydrolyzed; acetonitrile is used instead of ethanol to separate sugars from enzyme-resistant carbohydrates, proteins, and other macromolecules; and a solid-phase extraction filter is included to remove substances that interfere with high-performance liquid chromatography (HPLC). Recovery studies indicate a > 97% sugar recovery. Twenty foods were analyzed. After enzymatic hydrolysis, fructose, glucose, sucrose, maltose, and lactose were extracted and determined by HPLC using a refractive index detector. Starch content was calculated from the increase in the amount of glucose. The results were compared with values listed on the "Nutrition Facts" panel for that food. The analyzed amounts of sugars and starches were 73-96% of declared values.
开发了一种用于测定加工食品中糖类和淀粉的分离方案。它基于美国官方分析化学家协会（AOAC）方法985.29测定总膳食纤维，并做了以下修改：碳水化合物淀粉在水解前被分离成可溶和不溶部分；使用乙腈代替乙醇从抗酶解碳水化合物、蛋白质和其他大分子中分离糖类；并包含一个固相萃取过滤器以去除干扰高效液相色谱（HPLC）的物质。回收率研究表明糖类回收率>97%。分析了20种食品。酶解后，使用示差折光检测器通过HPLC提取并测定果糖、葡萄糖、蔗糖、麦芽糖和乳糖。根据葡萄糖量的增加计算淀粉含量。将结果与该食品“营养成分”标签上列出的值进行比较。糖类和淀粉的分析量为申报值的73 - 96%。
