Englyst H N, Cummings J H
M.R.C. Dunn Clinical Nutrition Centre, Cambridge, U.K.
J Assoc Off Anal Chem. 1988 Jul-Aug;71(4):808-14.
A method is described that allows rapid estimation of total, soluble, and insoluble dietary fiber as the non-starch polysaccharides (NSP) in plant foods. It is a modification of an earlier, more complex procedure. Starch is completely removed enzymatically, and NSP is measured as the sum of its constituent sugars released by acid hydrolysis. The sugars may, in turn, be measured by gas chromatography (GC), giving values for individual monosaccharides, or more rapidly by colorimetry. Both GC and colorimetry are suitable for routine measurement of total, soluble, and insoluble dietary fiber in cereals, fruits, and vegetables. Values obtained are not affected by food processing so the dietary fiber content of various processed foods and mixed diets can be calculated simply from knowing the amount in the raw materials. The additional information obtained by GC analysis is valuable in the interpretation of physiological studies and in epidemiology where disease is related to type and amount of dietary fiber.
本文描述了一种方法，该方法可快速估算植物性食物中作为非淀粉多糖（NSP）的总膳食纤维、可溶性膳食纤维和不溶性膳食纤维。它是对早期更复杂程序的一种改进。通过酶法完全去除淀粉，将NSP测定为酸水解释放的其组成糖的总和。这些糖可以依次通过气相色谱法（GC）进行测量，得出各个单糖的值，或者通过比色法更快速地测量。气相色谱法和比色法都适用于谷物、水果和蔬菜中总膳食纤维、可溶性膳食纤维和不溶性膳食纤维的常规测量。所获得的值不受食品加工的影响，因此各种加工食品和混合饮食的膳食纤维含量可以简单地根据原材料中的含量来计算。通过气相色谱分析获得的额外信息在生理学研究和流行病学解释中很有价值，在流行病学中，疾病与膳食纤维的类型和数量有关。
