Morgan L W, Van Bonn W, Jensen E D, Ridgway S H
School of Veterinary Medicine, University of Wisconsin, Madison 53706, USA.
J Zoo Wildl Med. 1999 Mar;30(1):70-5.
The effects of in vitro hemolysis on 23 biochemical analytes were assessed in sera from 14 clinically healthy Atlantic bottlenose dolphins (Tursiops truncatus). Each serum sample was divided into three portions for analysis: 1) nonhemolyzed control; 2) moderate hemolysis, simulated by adding hemolyzed serum to a final concentration of approximately 150 mg/dl Hb; and 3) severe hemolysis, simulated by adding hemolyzed serum to a final concentration of approximately 500 mg/dl Hb. Moderate hemolysis resulted in statistically significant increases in the mean values of iron, lactate dehydrogenase, potassium, and uric acid and a decrease in creatinine (P < 0.001). Severe hemolysis resulted in statistically significant changes in the mean values of the above analytes in addition to the following increases: alanine aminotransferase, calcium, and serum globulins (P < 0.001) and albumin and total protein (P < 0.01). Total bilirubin and gamma glutamyl transferase levels were lower in the severely hemolyzed sample (P < 0.001). Differences in mean values for alkaline phosphatase between nonhemolyzed and hemolyzed serum were not significant but did show a downward trend in the hemolyzed sera. The presence and severity of hemolysis must be considered in the interpretation of the serum chemistry values.
在14只临床健康的大西洋宽吻海豚（瓶鼻海豚）的血清中，评估了体外溶血对23种生化分析物的影响。每个血清样本被分成三份用于分析：1）未溶血对照；2）中度溶血，通过添加溶血血清至最终浓度约150mg/dl血红蛋白来模拟；3）重度溶血，通过添加溶血血清至最终浓度约500mg/dl血红蛋白来模拟。中度溶血导致铁、乳酸脱氢酶、钾和尿酸的平均值出现统计学上的显著升高，肌酐降低（P<0.001）。重度溶血除了上述分析物平均值出现统计学上的显著变化外，还导致以下指标升高：丙氨酸转氨酶、钙和血清球蛋白（P<0.001）以及白蛋白和总蛋白（P<0.01）。重度溶血样本中的总胆红素和γ-谷氨酰转移酶水平较低（P<0.001）。未溶血和溶血血清之间碱性磷酸酶平均值的差异不显著，但溶血血清中确实呈现下降趋势。在解释血清化学值时，必须考虑溶血的存在和严重程度。
