Levy Julie K, Crawford P Cynda, Werner Linda L
Department of Small Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL 32610, USA.
J Am Vet Med Assoc. 2006 Apr 1;228(7):1033-7. doi: 10.2460/javma.228.7.1033.
To determine the effect of age on reference intervals of serum biochemical values in kittens.
Prospective clinical trial.
55 kittens from 12 specific-pathogen-free queens.
Kittens were allocated at birth into colostrum-fed (n = 27) and colostrum-deprived (28) groups. Blood was collected at birth and on days 1, 2, 4, 7, 14, 28, and 56. Serum samples were analyzed for activities of alkaline phosphatase, alanine aminotransferase, aspartate aminotransferase, creatine kinase, lactate dehydrogenase, gamma-glutamyltransferase, amylase, and lipase and for concentrations of albumin, total protein, bilirubin, urea nitrogen, creatinine, cholesterol, glucose, calcium, phosphorus, and triglycerides by use of an automated analyzer. Total serum solids concentrations were determined by use of refractometry. Serum IgG concentrations were quantified by use of radial immunodiffusion.
For several analytes, reference intervals changed rapidly, most notably during the first few days of life. Reference intervals for alkaline phosphatase, creatine kinase, lactate dehydrogenase, and triglycerides were higher from birth to 8 weeks than adult reference intervals. Aspartate aminotransferase, bilirubin, urea nitrogen, and creatinine were higher than in adults at birth but were similar to or lower than adult reference intervals by 8 weeks. Compared with adult reference intervals, reference intervals for calcium and phosphorus concentrations were higher and for albumin and total protein concentrations were lower throughout the study period.
Important differences exist between reference intervals for serum biochemical values of neonatal and adult cats. Age-appropriate reference intervals should be used for accurate assessment of serum biochemical test results in cats.
确定年龄对小猫血清生化值参考区间的影响。
前瞻性临床试验。
来自12只无特定病原体母猫的55只小猫。
小猫出生时被分为初乳喂养组（n = 27）和初乳剥夺组（28只）。在出生时以及出生后第1、2、4、7、14、28和56天采集血液。使用自动分析仪分析血清样本中的碱性磷酸酶、丙氨酸氨基转移酶、天冬氨酸氨基转移酶、肌酸激酶、乳酸脱氢酶、γ-谷氨酰转移酶、淀粉酶和脂肪酶的活性，以及白蛋白、总蛋白、胆红素、尿素氮、肌酐、胆固醇、葡萄糖、钙、磷和甘油三酯的浓度。通过折射法测定血清总固体浓度。使用放射免疫扩散法定量血清IgG浓度。
对于几种分析物，参考区间变化迅速，最明显的是在生命的最初几天。从出生到8周，碱性磷酸酶、肌酸激酶、乳酸脱氢酶和甘油三酯的参考区间高于成年参考区间。出生时天冬氨酸氨基转移酶、胆红素、尿素氮和肌酐高于成年猫，但到8周时与成年参考区间相似或更低。与成年参考区间相比，在整个研究期间，钙和磷浓度的参考区间较高，而白蛋白和总蛋白浓度的参考区间较低。
新生猫和成年猫血清生化值的参考区间存在重要差异。应使用适合年龄的参考区间来准确评估猫的血清生化检测结果。
