Ingram G I, Hills M
Thromb Haemost. 1976 Aug 31;36(1):230-6.
Blood samples from 12 normal subjects and 46 patients on oral anticoagulants were divided so that each was anticoagulated with four concentrations of trisodium citrate solution in the range 0.09-0.15 M, 9 vol blood being added to 1 vol citrate solution in each case. Citrated blood haematocrit and plasma prothrombin time was measured on each subsample; 0.025 M CaCl2-solution was used for recalcification throughout. Three laboratories participated, each using a different prothrombin-time technique.
从12名正常受试者和46名服用口服抗凝剂的患者采集血样，并进行分组，使每组血样分别用浓度范围为0.09 - 0.15M的四种柠檬酸钠溶液进行抗凝处理，每种情况下均按9体积血液加1体积柠檬酸盐溶液的比例添加。对每个子样本测量枸橼酸化血液的血细胞比容和血浆凝血酶原时间；整个过程均使用0.025M氯化钙溶液进行重新钙化。三个实验室参与了此项研究，每个实验室使用不同的凝血酶原时间检测技术。
