Wang N L, Tai F H, Chung J S, Chen C P
Zhonghua Min Guo Wei Sheng Wu Xue Za Zhi. 1978 Jun;11(2):39-44.
A method for rapidly identifying and quantitating small amount of staphylococcal enterotoxin B was developed on capillary tube single diffusion test. The high sensitivity of method has the advantages to save antiserum and incubation period. Some factors such as concentration of antigen and antiserum, incubation temperature, buffer systems, and position of capillary tube all might affect the test accuracy. If all those affecting factors were concerned, the toxin would probably be standardized. The most accessible quantitative estimation was accomplished by means of (1) plotting a precipitation curve from the different concentrations of standardized staphylococcal enterotoxin B; (2) the sample to be test was diluted by serial two-fold method with the same buffer system; (3) select suitable 3--5 dilutions to perform capillary tube diffusion test, taking the values of precipitation bands as abcissa and sample dilutions as longitudinal axis, rendered the precipitation curve of sample exquisitely paralled to that of the standard; (4) calibrate the figure values of sample exactly on the precipitation curve, and reading the toxin content of specimen to be tested from standard curve.
建立了一种基于毛细管单向扩散试验快速鉴定和定量检测少量葡萄球菌肠毒素B的方法。该方法灵敏度高，具有节省抗血清和缩短孵育时间的优点。抗原和抗血清浓度、孵育温度、缓冲体系以及毛细管位置等因素均可能影响检测准确性。若考虑所有这些影响因素，毒素检测可能实现标准化。最简便的定量评估方法如下：（1）用不同浓度的标准化葡萄球菌肠毒素B绘制沉淀曲线；（2）用相同缓冲体系将待测样品按两倍系列稀释法进行稀释；（3）选择合适的3 - 5个稀释度进行毛细管扩散试验，以沉淀带数值为横坐标、样品稀释度为纵坐标，使样品沉淀曲线与标准曲线完美平行；（4）在沉淀曲线上精确校准样品的数值，并从标准曲线读取待测标本的毒素含量。
