Cingolani M, Froldi R, Mencarelli R, Rodriguez D
Institute of Legal Medicine, University of Ancona, Ospedale Regionale, Italy.
J Anal Toxicol. 1999 May-Jun;23(3):219-21. doi: 10.1093/jat/23.3.219.
This study reports the results of the detection and quantitation of strychnine in formalin-fixed tissues and in the formalin solutions in which the tissues were fixed. The toxicological analyses were performed on formalin-fixed liver and kidney samples and formalin solutions (10% buffered pH 7) in which the same samples from a case of acute strychnine poisoning were preserved. The analyses carried out at the time of autopsy on body fluid and tissues (bile, 2.40 mg/L; stomach contents, 14.2 mg; liver, 6.68 mg/kg; kidney, 2.68 mg/kg) allowed the identification of this substance as cause of death. The tissue samples were preserved in formalin solutions for 8 weeks. The analyses performed on formalin-fixed tissues (liver and kidney) and on formalin solutions, in which the same tissues were preserved, permitted the detection and quantitation of strychnine (liver, 1.59 mg/kg; formalin from the liver, 1.80 mg/L; kidney, 0.98 mg/kg; formalin from the kidney, 1.11 mg/L). The results indicate that this particular toxic substance also shows good stability in biological specimens subjected to chemical fixation.
本研究报告了在福尔马林固定组织及组织固定所用福尔马林溶液中士的宁的检测与定量结果。对福尔马林固定的肝脏和肾脏样本以及福尔马林溶液（10%，pH 7缓冲）进行了毒理学分析，这些样本来自一例急性士的宁中毒病例。尸检时对体液和组织（胆汁，2.40毫克/升；胃内容物，14.2毫克；肝脏，6.68毫克/千克；肾脏，2.68毫克/千克）进行的分析确定该物质为死因。组织样本在福尔马林溶液中保存了8周。对福尔马林固定组织（肝脏和肾脏）以及保存这些组织的福尔马林溶液进行的分析，实现了士的宁的检测与定量（肝脏，1.59毫克/千克；肝脏福尔马林，1.80毫克/升；肾脏，0.98毫克/千克；肾脏福尔马林，1.11毫克/升）。结果表明，这种特定的有毒物质在经过化学固定的生物标本中也具有良好的稳定性。
