Cingolani M, Froldi R, Mencarelli R, Mirtella D, Rodriguez D
Institute of Legal Medicine, University of Ancona, Ospedale Regionale, I-60020, Italy.
J Anal Toxicol. 2001 Jan-Feb;25(1):31-4. doi: 10.1093/jat/25.1.31.
The study reports the results of the detection and quantitation of morphine in tissues fixed in formalin and in the formalin solutions in which the same tissues were fixed. Toxicological analyses were performed on formalin-fixed liver and kidney samples from five cases of opiate poisoning and on the formalin solutions (10% buffered pH 7) in which the samples were preserved. Analyses carried out at the time of autopsy on body fluids and tissues allowed identification of opiate as the cause of death and its quantitation. Tissue samples were preserved in formalin solutions for 12 weeks before analysis. The mean levels of recovery of morphine in fixed tissues were 36.29% in liver, 29.41% in kidney, 74.93% in formalin from liver, and 42.17% in formalin from kidney. Results indicated that this particular toxic substance shows good stability even in biological specimens subjected to chemical fixation.
该研究报告了在福尔马林固定的组织以及固定这些组织所用的福尔马林溶液中吗啡的检测和定量结果。对来自5例阿片类药物中毒病例的福尔马林固定的肝脏和肾脏样本以及保存这些样本的福尔马林溶液（10%，pH 7缓冲）进行了毒理学分析。尸检时对体液和组织进行的分析能够确定阿片类药物为死因并对其进行定量。组织样本在分析前于福尔马林溶液中保存12周。固定组织中吗啡的平均回收率在肝脏中为36.29%，在肾脏中为29.41%，肝脏福尔马林中为74.93%，肾脏福尔马林中为42.17%。结果表明，这种特定的有毒物质即使在经过化学固定的生物标本中也具有良好的稳定性。
