Lengeler K B, Kothe E
Friedrich-Schiller-Universität, Biologisch-Pharmazeutische Fakultät, Institut für Mikrobiologie, Mikrobielle Phytopathologie, Beutenbergstrasse 11, D-07745 Jena, Germany.
Curr Genet. 1999 Jun;35(5):551-6. doi: 10.1007/s002940050452.
To identify genes regulated by the B mating-type genes a differential RNA display was established for the homobasidiomycete Schizophyllum commune and a gene, brt1, was identified. The mRNA concerned is highly abundant in monokaryotic mycelia and is down-regulated in matings in which either the B genes alone or else both the A and B genes are different between the mates. This places the gene brt1 under the control of the pheromone response system of S. commune encoded by the B mating-type loci. Sequence analysis revealed similarity to a novel protein family, two members of which have been shown to inhibit translational re-initiation.
为了鉴定受B交配型基因调控的基因，建立了一种针对同担子菌裂褶菌的差异RNA显示方法，并鉴定出一个基因brt1。相关mRNA在单核菌丝体中高度丰富，在交配过程中会下调，在这些交配中，要么仅B基因不同，要么交配双方的A和B基因均不同。这表明brt1基因受裂褶菌由B交配型位点编码的信息素反应系统的控制。序列分析显示与一个新的蛋白质家族相似，该家族的两个成员已被证明可抑制翻译重新起始。
