Fowler Thomas J, Mitton Michael F, Rees Eleanor I, Raper Carlene A
Department of Microbiology and Molecular Genetics, University of Vermont, 208 Stafford Hall, 95 Carrigan Drive, Burlington, VT 05405-0001, USA.
Fungal Genet Biol. 2004 Jan;41(1):89-101. doi: 10.1016/j.fgb.2003.08.009.
In this study, genes of the Schizophyllum commune Balpha and Bbeta mating-type loci are shown to be within a few kilobases of each other. The region between the nearest Balpha and Bbeta genes contains many short direct repeats. Predicted amino acid sequences and activity spectra of three pheromones encoded in the Balpha3 mating-type specificity are presented along with a re-evaluation of pheromone activity of many previously reported S. commune lipopeptide pheromones. This analysis showed that S. commune pheromones belong to five subtypes. Several pheromones activate both a Bbeta receptor and a Balpha receptor, a phenomenon previously unrecognized. Clues from mating tests and DNA hybridization led to the cloning of bar8, the gene encoding the Balpha8 pheromone receptor, Bar8. Bar8 is similar in sequence to Bbr1, the Bbeta1 pheromone receptor, and functionally identical to it. These data begin to elucidate the enigmatic recombination patterns previously encountered at the B mating-type complex.
在本研究中，裂褶菌（Schizophyllum commune）Bα和Bβ交配型位点的基因彼此相距仅几千碱基对。最近的Bα和Bβ基因之间的区域包含许多短的直接重复序列。给出了Bα3交配型特异性中编码的三种信息素的预测氨基酸序列和活性谱，并对许多先前报道的裂褶菌脂肽信息素的信息素活性进行了重新评估。该分析表明，裂褶菌信息素属于五个亚型。几种信息素既能激活Bβ受体，也能激活Bα受体，这是一种以前未被认识到的现象。交配试验和DNA杂交的线索导致了bar8的克隆，bar8是编码Bα8信息素受体Bar8的基因。Bar8在序列上与Bβ1信息素受体Bbr1相似，并且在功能上与其相同。这些数据开始阐明先前在B交配型复合体中遇到的神秘重组模式。
