Beesley A H, Qureshi I Z, Giesberts A N, Parker A J, White S J
Laboratory for Membrane Protein Function, Department of Biomedical Science, The University of Sheffield, Western Bank, Sheffield S10 2TN, UK.
Pflugers Arch. 1999 Jun;438(1):1-7. doi: 10.1007/s004240050872.
The sulphonylurea receptor (SUR) is the site of action for sulphonylurea derivatives such as glibenclamide, which are widely used as oral hypoglycaemic agents. Sulphonylureas have also been shown to affect urine flow and salt excretion by the kidney; therefore, the use of these drugs may have important implications for the pharmacological manipulation of renal salt handling. The purpose of the present investigation was to increase our understanding of the possible role of SUR in the regulation of renal function by determining the distribution of SUR isoforms within mouse kidney. Immunostaining with anti-SUR antisera revealed specific staining of SUR2B in distal nephron segments of mouse kidney. A diffuse, low level staining was observed in proximal tubules in the inner cortical region. No evidence was found for the presence of SUR2B in intra-renal blood vessels. Reverse-transcription polymerase chain reaction and Western blotting experiments indicated that SUR2B is the only known isoform expressed. These data demonstrate that SUR2B in mouse kidney is expressed in tubule regions that are critical in determining renal salt excretion.
磺脲类受体（SUR）是格列本脲等磺脲类衍生物的作用位点，这些衍生物被广泛用作口服降糖药。磺脲类药物也已被证明会影响肾脏的尿流和盐分排泄；因此，使用这些药物可能对肾脏盐分处理的药理学调控具有重要意义。本研究的目的是通过确定SUR亚型在小鼠肾脏中的分布，加深我们对SUR在肾功能调节中可能作用的理解。用抗SUR抗血清进行免疫染色显示，SUR2B在小鼠肾脏远曲小管段有特异性染色。在内皮质区域的近端小管中观察到弥漫性、低水平染色。未发现肾内血管中有SUR2B存在的证据。逆转录聚合酶链反应和蛋白质印迹实验表明，SUR2B是唯一已知表达的亚型。这些数据表明，小鼠肾脏中的SUR2B在对确定肾脏盐分排泄至关重要的肾小管区域表达。
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