Rodríguez S, Navarrete E H, Vio K, González C, Schöbitz K, Rodríguez E M
Instituto de Histología y Patología, Facultad de Medicina, Universidad Austral de Chile, Valdivia, Chile.
Cell Tissue Res. 1999 Jun;296(3):457-69. doi: 10.1007/s004410051306.
The subcommissural organ (SCO) secretes glycoproteins into the cerebrospinal fluid (CSF) that aggregate and form Reissner's fiber (RF). The factors involved in this aggregation are not known. One factor may be the hydrodynamics of the CSF when flowing through the aqueduct. This hypothesis was tested by isografting rat SCO and xenografting bovine SCO into the lateral ventricle of rats. Xenografts were either fresh bovine SCO or explants cultured for 30 days before transplantation. The grafts were investigated by electron microscopy and immunocytochemistry using antibodies against RF glycoproteins, serotonin and the glucose transporter I. Maximal time of transplantation was 43 days for isografts and 14 days for xenografts. The isografts were not reinnervated but were revascularized; they secreted into the ventricle RF glycoproteins that became progressively packed into pre-RF and RF structures identical to those formed by the SCO in situ. RF was confined to the host ventricle and at its distal end the constituent proteins disassembled. Xenografts were neither reinnervated nor revascularized and secreted into the host ventricle a material that never formed an RF. These findings indicate that the CSF factor responsible for the formation of RF is species specific, and that this process does not depend on the hydrodynamics of the CSF. The blood vessels revascularizing the isografted SCO acquired the characteristics of the vessels irrigating the SCO in situ, namely, a tight endothelium displaying glucose transporter I, and a perivascular space containing long-spacing collagen, thus indicating that basal release of glycoproteins may also occur in the grafted SCO.
连合下器官（SCO）向脑脊液（CSF）中分泌糖蛋白，这些糖蛋白聚集并形成赖氏纤维（RF）。参与这种聚集的因素尚不清楚。其中一个因素可能是脑脊液流经导水管时的流体动力学。通过将大鼠SCO同基因移植以及将牛SCO异种移植到大鼠侧脑室来验证这一假说。异种移植的材料要么是新鲜的牛SCO，要么是移植前培养30天的外植体。使用针对RF糖蛋白、5-羟色胺和葡萄糖转运体I的抗体，通过电子显微镜和免疫细胞化学对移植物进行研究。同基因移植的最长时间为43天，异种移植为14天。同基因移植的移植物没有重新神经支配，但有血管再生；它们向脑室分泌RF糖蛋白，这些糖蛋白逐渐堆积形成与原位SCO形成的前RF和RF结构相同的结构。RF局限于宿主脑室，在其远端，组成蛋白会分解。异种移植的移植物既没有重新神经支配也没有血管再生，并且向宿主脑室分泌一种从未形成RF的物质。这些发现表明，负责RF形成的脑脊液因子具有物种特异性，并且这个过程不依赖于脑脊液的流体动力学。为同基因移植的SCO提供血管再生的血管获得了原位为SCO供血的血管的特征，即具有紧密内皮且显示葡萄糖转运体I，以及含有长间距胶原的血管周围间隙，因此表明移植的SCO中也可能发生糖蛋白的基础释放。
