Rodríguez E M, Rodríguez S, Schoebitz K, Yulis C R, Hoffmann P, Manns V, Oksche A
Instituto de Histología y Patología, Universidad Austral de Chile, Valdivia.
Cell Tissue Res. 1989 Dec;258(3):499-514. doi: 10.1007/BF00218862.
There is increasing evidence that, in the rat, a serotonin-mediated neural input may have an inhibitory influence on the secretory activity of the subcommissural organ (SCO). In the present investigation the rat SCO was studied 7, 30 and 90 days after transplantation under the kidney capsule, an area devoid of local serotonin-containing nerves. The grafted tissue was examined by use of immunocytochemistry employing a series of primary antisera, lectin histochemistry and transmission electron microscopy. The grafted SCO survived transplantation and contained, in addition to secretory ependymal and hypendymal SCO-cells, also elements immunoreactive with antisera against glial fibrillary acidic protein or S-100 protein. In transplants, SCO-cells produced a material displaying the characteristic immunocytochemical and lectin-binding properties of SCO-cells observed under in-situ conditions. The ependymal cells lined 1-3 small cavities, which contained secretory material. A fully developed structural equivalent of Reissner's fiber was, however, never found. The immunocytochemical and ultrastructural study of the grafted SCO showed an absence of nerve fibers within the graft and suggested a state of enhanced secretory activity. A network of protruding basal lamina structures connected the secretory cells to the newly formed capillaries revascularizing the SCO. One week after transplantation, long-spacing collagen started to appear in expanded areas of such laminar networks and also in the perivascular space. It is suggested (i) that the formation of long-spacing forms of collagen is triggered by factors provided by the SCO-secretory cells, and (ii) that secretory material of the ependymal and hypendymal cells may reach the reticular extensions of the basal lamina. In contrast to the SCO in situ, the grafted SCO-cells showed a positive immunoreaction for neuron-specific enolase. They became surrounded by a S-100-immunoreactive glial sheath that separated them from other transplanted cell types and the adjacent kidney tissue of the host.
越来越多的证据表明，在大鼠中，5-羟色胺介导的神经输入可能对室管膜下器官（SCO）的分泌活动产生抑制作用。在本研究中，对移植到肾被膜下（一个不含局部含5-羟色胺神经的区域）7天、30天和90天后的大鼠SCO进行了研究。通过使用一系列一抗的免疫细胞化学、凝集素组织化学和透射电子显微镜对移植组织进行了检查。移植的SCO在移植后存活，除了分泌性室管膜和室管膜下SCO细胞外，还含有与抗胶质纤维酸性蛋白或S-100蛋白的抗血清发生免疫反应的成分。在移植组织中，SCO细胞产生一种物质，其显示出在原位条件下观察到的SCO细胞的特征性免疫细胞化学和凝集素结合特性。室管膜细胞排列在1 - 3个小腔内，腔内含有分泌物质。然而，从未发现完全发育的瑞氏纤维的结构等同物。对移植的SCO的免疫细胞化学和超微结构研究显示移植组织内没有神经纤维，并提示分泌活动增强的状态。突出的基膜结构网络将分泌细胞与使SCO重新血管化的新形成的毛细血管连接起来。移植后一周，长间距胶原开始出现在这种层状网络的扩大区域以及血管周围间隙中。有人提出：（i）长间距胶原形式的形成是由SCO分泌细胞提供的因子触发的；（ii）室管膜和室管膜下细胞的分泌物质可能到达基膜的网状延伸部分。与原位SCO不同，移植的SCO细胞对神经元特异性烯醇化酶呈阳性免疫反应。它们被一个S-100免疫反应性胶质鞘包围，该鞘将它们与其他移植细胞类型和宿主的相邻肾组织分隔开来。
