Pantarotto C, Belvedere G, Frigerio A
Arzneimittelforschung. 1976;26(10):1857-60.
A method to measure the alpha-sympathomimetic amine, 1-(3,5-dimethoxy-4-hydroxyphenyl)-2-monomethyl-amino-ethanol (dimethophrine, DMP), was developed and applied for the determination of the drug in biological specimens. The gas chromatographic degradation of the drug was investigated, and in order to avoid this process, DMP is converted to the trifluoroacetyl derivative and assayed by gas liquid chromatography on an OV 1' column. The minimum detectable amount is 25 pg per injection. Linearity (using hexachlorobenzene as internal standard for quantitation) was found up to mug amounts of the substance, without any interference with endogenous substrates. Values from plasma, red blood cells and urine in rabbits treated with a single dose of DMP (10 mg/kg) are also reported.
已开发出一种测量α-拟交感神经胺1-(3,5-二甲氧基-4-羟基苯基)-2-单甲基氨基乙醇（二甲福林，DMP）的方法，并将其应用于生物样品中该药物的测定。对该药物的气相色谱降解进行了研究，为避免此过程，将DMP转化为三氟乙酰衍生物，并在OV 1'柱上通过气液色谱法进行测定。每次进样的最低检测量为25 pg。（以六氯苯作为定量内标）发现该物质在微克量范围内呈线性，且不受内源性底物的干扰。还报告了用单剂量DMP（10 mg/kg）处理的兔子血浆、红细胞和尿液中的值。
