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BseSI, a restriction endonuclease from Bacillus stearothermophilus Jo 10-553, which recognizes the novel hexanucleotide sequence 5'-G(G/T)GC(A/C)C-3'.BseSI是一种来自嗜热脂肪芽孢杆菌Jo 10-553的限制性内切酶,它识别新的六核苷酸序列5'-G(G/T)GC(A/C)C-3'。
Nucleic Acids Res. 1999 Jul 1;27(13):2644-5. doi: 10.1093/nar/27.13.2644.
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BseSI是一种来自嗜热脂肪芽孢杆菌Jo 10-553的限制性内切酶,它识别新的六核苷酸序列5'-G(G/T)GC(A/C)C-3'。

BseSI, a restriction endonuclease from Bacillus stearothermophilus Jo 10-553, which recognizes the novel hexanucleotide sequence 5'-G(G/T)GC(A/C)C-3'.

作者信息

Steponaviciene D, Maneliene Z, Petrusyte M, Janulaitis A

机构信息

MBI Fermentas, Institute of Biotechnology, Graiciũno 8, 2028 Vilnius, Lithuania.

出版信息

Nucleic Acids Res. 1999 Jul 1;27(13):2644-5. doi: 10.1093/nar/27.13.2644.

DOI:10.1093/nar/27.13.2644
PMID:10373580
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC148472/
Abstract

A new restriction endonuclease Bse SI has been isolated from Bacillus stearothermophilus Jo10-553. Bse SI recognizes a degenerate hexanucleotide sequence 5'-G(G/T)GC(A/C)C-3' and cleaves DNA to produce 3[prime]-protruding tetranucleotide ends.

摘要

一种新的限制性内切酶Bse SI已从嗜热脂肪芽孢杆菌Jo10 - 553中分离出来。Bse SI识别一个简并六核苷酸序列5'-G(G/T)GC(A/C)C-3',并切割DNA产生3'端突出的四核苷酸末端。