一氧化氮可逆转前列腺素对大鼠卵巢孕酮分泌的抑制作用。

Nitric oxide reverses prostaglandin-induced inhibition in ovarian progesterone secretion in rats.

作者信息

Dong Y L, Gangula P R, Fang L, Yallampalli C

机构信息

Department of Obstetrics and Gynecology, University of Texas Medical Branch, Galveston 77555, USA.

出版信息

Hum Reprod. 1999 Jan;14(1):27-32. doi: 10.1093/humrep/14.1.27.

DOI:10.1093/humrep/14.1.27

PMID:10374089

Abstract

The present studies were undertaken to determine whether nitric oxide (NO) is involved in the regulation of ovarian progesterone and oestradiol secretion in rats. Immature female Sprague-Dawley rats at 27 days of age were injected s.c. with 4 IU pregnant mare's serum gonadotrophin (PMSG) and were killed 72 h after the injection. The ovaries were collected, weighed and cultured in Dulbecco's modified Eagle's medium containing saline, NO donor, NO synthesis inhibitor or prostaglandin F2 alpha (PGF2 alpha). After 24 h culture, the medium concentrations of progesterone and oestradiol were measured by radioimmunoassay. Results showed that: (i) diethylenetriamine (DETA)/NO (1 x 10(-6), 1 x 10(-5), 1 x 10(-4) M), an NO donor, caused a dose-dependent increase in progesterone synthesis (355 +/- 43, 443 +/- 46, 647 +/- 55 ng/g ovary respectively, P < 0.01) with a concomitant decrease in ovarian oestradiol secretion (408.1 +/- 50.7, 272.9 +/- 28.2, 132.6 +/- 34.6 pg/g ovary respectively, P < 0.01); (ii) neither progesterone nor oestradiol concentrations in the culture medium were altered by DETA without NO; (iii) NG-nitro-l-arginine methyl ester (1 x 10(-4) M), an inhibitor of NO synthesis, did not significantly affect progesterone and oestradiol secretion by rat ovaries; (iv) PGF2 alpha(1 x 10(-6) M) caused a fall in progesterone and oestradiol synthesis; (v) co-incubation with DETA/NO, significantly reversed the PGF2 alpha-induced decrease in progesterone concentrations from 184 +/- 29 to 388 +/- 60 ng/g (P < 0.01), but not that of oestradiol. It can be concluded that NO up-regulates progesterone secretion and down-regulates oestradiol secretion in rat ovaries, and NO can reverse PGF2 alpha-induced inhibition in ovarian progesterone secretion.

摘要

本研究旨在确定一氧化氮（NO）是否参与大鼠卵巢孕酮和雌二醇分泌的调节。27日龄的未成熟雌性斯普拉格-道利大鼠皮下注射4国际单位孕马血清促性腺激素（PMSG），并在注射后72小时处死。收集卵巢，称重并在含有生理盐水、NO供体、NO合成抑制剂或前列腺素F2α（PGF2α）的杜氏改良 Eagle 培养基中培养。培养24小时后，通过放射免疫测定法测量培养基中孕酮和雌二醇的浓度。结果显示：（i）NO供体二亚乙基三胺（DETA）/NO（1×10⁻⁶、1×10⁻⁵、1×10⁻⁴M）导致孕酮合成呈剂量依赖性增加（分别为355±43、443±46、647±55 ng/g卵巢，P<0.01），同时卵巢雌二醇分泌减少（分别为408.1±50.7、272.9±28.2、132.6±34.6 pg/g卵巢，P<0.01）；（ii）不含NO的DETA不会改变培养基中孕酮和雌二醇的浓度；（iii）NO合成抑制剂NG-硝基-L-精氨酸甲酯（1×10⁻⁴M）对大鼠卵巢孕酮和雌二醇分泌无显著影响；（iv）PGF2α（1×10⁻⁶M）导致孕酮和雌二醇合成减少；（v）与DETA/NO共同孵育可显著逆转PGF2α诱导的孕酮浓度下降，从184±29降至388±60 ng/g（P<0.01），但不能逆转雌二醇的下降。可以得出结论，NO上调大鼠卵巢孕酮分泌并下调雌二醇分泌，并且NO可以逆转PGF2α诱导的卵巢孕酮分泌抑制。