Li Y, Qiu J
Department of Pathology, Sun Yet-San University of Medical Science, Guangzhou.
Zhonghua Bing Li Xue Za Zhi. 1997 Oct;26(5):262-5.
To study the association between RB gene and the oncogenesis of bone tumors.
Southern blot and immunohistochemical techniques were used to detect the structural anomalies of RB gene in 34 cases of bone tumors and the expression of RB protein in 99 paraffin-embedded bone neoplasma.
The deletion and/or rearrangement of RB gene were detected only in 42.9% (9/21) of osteosarcoma; lack of RB protein expression was noticed in 12 cases including 7 cases of osteosarcoma (7/26, 26.9%) and 5 cases of chondrosarcoma (5/23, 21.7%). Beniga giant cell tumor of bone and chondroblastoma showed positive RB protein expression; osteosarcoma cells presenting poor differentiation and apparent atypia all showed no expression of RB protein. Most of the high-grade chondrosarcoma also had no RB protein expression.
The alterations of RB gene and loss of RB protein may play a role in the pathogenesis and progression of malignant bone neoplasms.
研究RB基因与骨肿瘤发生的关系。
采用Southern印迹法和免疫组化技术检测34例骨肿瘤中RB基因的结构异常及99例石蜡包埋骨肿瘤组织中RB蛋白的表达。
仅在42.9%（9/21）的骨肉瘤中检测到RB基因的缺失和/或重排；12例出现RB蛋白表达缺失，其中包括7例骨肉瘤（7/26，26.9%）和5例软骨肉瘤（5/23，21.7%）。骨巨细胞瘤和软骨母细胞瘤呈RB蛋白表达阳性；分化差、异型性明显的骨肉瘤细胞均无RB蛋白表达。大多数高级别软骨肉瘤也无RB蛋白表达。
RB基因改变和RB蛋白缺失可能在恶性骨肿瘤的发病机制和进展中起作用。
