Zhe X, Chen J, Liu T
Department of Pathology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing.
Zhonghua Bing Li Xue Za Zhi. 1997 Oct;26(5):289-92.
To elucidate the relationship between bcl-2 gene expression and the frequency of apoptosis of tumor cells, and study the expression of p16 tumor suppressor gene in neuroblastoma.
In situ hybridization and immunohistochemistry methods were used to study the frequencies of expression of bcl-2 and p16 genes in 20 cases of neuroblastoma. Meanwhile, an in situ apoptotic cell detection method was adopted to detect the apoptotic cells in these tumors, and the number of apoptotic cells was compared with the bcl-2 gene expression in each case.
In situ hybridization revealed that the positive frequencies of both bcl-2 and p16 gene expression in 20 neuroblastoma specimens were 95%, and the expression rates at the protein level of these 2 gene products as detected by immunohistochemistry were both 100%. There was no significant difference between the positive rates obtained by these 2 methods. Comparing the bcl-2 expression and apoptotic cell number in these specimens, we found that the apoptotic cell number increased as the level of bcl-2 expression decreased.
The bcl-2 gene was expressed in most of human neuroblastomas. The reverse correlation of bcl-2 expression and tumor cell apoptosis further confirms that bcl-2 as an important gene inhibiting cell apoptosis may indirectly promote the carcinogenesis of neuroblastoma. It seems that there was no significant loss of p16 gene expression in these cases.
阐明bcl-2基因表达与肿瘤细胞凋亡频率之间的关系，并研究神经母细胞瘤中p16抑癌基因的表达情况。
采用原位杂交和免疫组化方法研究20例神经母细胞瘤中bcl-2和p16基因的表达频率。同时，采用原位凋亡细胞检测方法检测这些肿瘤中的凋亡细胞，并将每例凋亡细胞数量与bcl-（此处原文有误，应为bcl-2）基因表达情况进行比较。
原位杂交显示，20例神经母细胞瘤标本中bcl-2和p16基因表达的阳性频率均为95%，免疫组化检测这两种基因产物的蛋白水平表达率均为100%。这两种方法获得的阳性率之间无显著差异。比较这些标本中bcl-2表达与凋亡细胞数量，我们发现随着bcl-2表达水平降低，凋亡细胞数量增加。
bcl-2基因在大多数人类神经母细胞瘤中表达。bcl-2表达与肿瘤细胞凋亡的负相关进一步证实，bcl-2作为抑制细胞凋亡的重要基因可能间接促进神经母细胞瘤的发生。在这些病例中似乎没有明显的p16基因表达缺失。
