Zhe X, Chen J, Liu T, Zhang L, Li P, Wang D
Department of Pathology, Peking Union Medical College Hospital, National Laboratory of Medical Molecular Biology, CAMS & PUMC, Beijing 100730.
Chin Med Sci J. 1999 Jun;14(2):102-6.
N-myc gene amplification is the most characteristic feature of neuroblastoma. c-myc oncogene, another member of myc gene family, plays an important role in cell proliferation and differentiation. Both of them may contribute to tumorigenesis of neuroblastoma. In this study we use the in situ hybridization and immunocytochemical methods to test the frequencies of N-myc and c-myc expressions in 20 cases of human neuroblastoma at mRNA and protein levels. The positive rates of the expression of N-myc are 90% and 100% detected by in situ hybridization and immunocytochemical methods respectively. The positive rates of c-myc are 80% and 85% respectively. Sixty percent of the 20 specimens tested by in situ hybridization and 55% by immunocytochemistry show an inverse relationship between the expressions of these two oncogenes and this may indicate that there are different gene expression controlling mechanisms in different cases.
N-myc基因扩增是神经母细胞瘤最具特征性的表现。c-myc癌基因是myc基因家族的另一个成员，在细胞增殖和分化中起重要作用。它们两者都可能促成神经母细胞瘤的肿瘤发生。在本研究中，我们采用原位杂交和免疫细胞化学方法，在mRNA和蛋白质水平检测20例人类神经母细胞瘤中N-myc和c-myc的表达频率。通过原位杂交和免疫细胞化学方法检测到的N-myc表达阳性率分别为90%和100%。c-myc的阳性率分别为80%和85%。在通过原位杂交检测的20个标本中，60%以及通过免疫细胞化学检测的55%显示这两种癌基因的表达呈负相关，这可能表明不同病例存在不同的基因表达调控机制。
